Overview

  • Product name

  • Description

    Rabbit polyclonal to Fez1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Full length protein, corresponding to amino acids 1-392 of Human Fez1 (NP_005094.1).

  • Positive control

    • Recombinant Human Fez1 protein (ab132344) can be used as a positive control in WB. Fez1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab168919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.

Target

  • Function

    May be involved in axonal outgrowth as component of the network of molecules that regulate cellular morphology and axon guidance machinery. Able to restore partial locomotion and axonal fasciculation to C.elegans unc-76 mutants in germline transformation experiments. May participate in the transport of mitochondria and other cargos along microtubules.
  • Tissue specificity

    Mainly expressed in brain.
  • Sequence similarities

    Belongs to the zygin family.
  • Post-translational
    modifications

    Phosphorylated by protein kinase C zeta; which enhances interaction with UBE4B and polyubiquitination.
    Polyubiquitinated in a UBE4B-dependent manner; which does not lead to proteasomal degradation and may be important for neurogenic activity. Polyubiquitin linkage seems to be mainly through Lys-26.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Cell membrane. Co-localizes with both, alpha- and gamma-tubulin. Translocated from the plasma membrane to the cytoplasm by activation of the PKC zeta.
  • Information by UniProt
  • Database links

  • Alternative names

    • Fasciculation and elongation protein zeta 1 antibody
    • Fasciculation and elongation protein zeta-1 antibody
    • Fez 1 antibody
    • Fez antibody
    • FEZ1 antibody
    • FEZ1_HUMAN antibody
    • Fezl antibody
    • Zygin 1 antibody
    • Zygin I antibody
    • Zygin-1 antibody
    • Zygin1 antibody
    • ZyginI antibody
    see all

Images

  • All lanes : Anti-Fez1 antibody (ab168919) at 1 µg/ml

    Lane 1 : Fez1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa

References

ab168919 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
10 µg
Specification
Brain
Blocking step
BSA as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Ansley Kunnath

Verified customer

Submitted Jul 26 2017

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