Overview

  • Product name

    FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate
  • Description

    Novel pH responsive FFN. Measures localization and activity of dopaminergic presynaptic terminals.
  • Biological description

    Novel, pH responsive fluorescent false neurotransmitter (FFN). Rodent DAT and VMAT2 substrate. Enables two-photon microscopic imaging of localization and activity of dopaminergic presynaptic terminals in the striatum of mouse acute brain slice. More selective for dopaminergic synapses than FFN511 (ab120331).
    Exhibits greater fluorescence emission in neutral than acidic environments allowing optical measurement of synaptic vesicle content release.
    Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy and standard fluorescent microscopy. Compatible with GFP tags.
  • Purity

    > 98%
  • CAS Number

    1234064-11-9
  • Chemical structure

    Chemical Structure

Properties

Images

  • All images provided by Thorsten Lau

    Figure 1: Two neuronal cells stained with 50 μM FFN102 on differentiation day 10. Shown is a sum projection of a confocal z-stack. Accumulation of FFN102 can be observed along the neurites (arrows) and the cell soma (S).

  • Figure 2a: Images in the first row show a group of neuronal cells stained with 50 μM FFN102 (sum projection of a confocal stack). FFN102 localizes to structures on the cell soma (S) as well as neurites (arrows). Z indicates the area zoomed in for an additional z-stack. 

  • Figure 2b: Zoomed in area of “Z” from figure 2b. The arrow indicates a globular structure on a neurite. S indicates FFN102 positive structures on the cell soma.
  • Figure 3: FFN102 dye uptake inhibition on addition of VMAT2 inhibitor RO4-1284

References

ab120866 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry. I forwarded your questions to our Abcam Biochemicals division, who sent the following reply:

"Unfortunately we are currently unable to provide any further detail about this product (ab120866). This product is being sold under an exclusive license by Abcam and the patent holders have not revealed any further information about this product as they are waiting for their paper to be published.

We would be happy to contact the authors to determine at what stage their paper submission is at and update the researcher accordingly. Alternatively we will try and find out if there is any more information that we can provide, although due to intellectual property and patent issues this is unlikely until the manuscript is accepted.

We have an alternative product available, FFN202, where more information is available. It may be that this of interest to the customer?

We will update you when the paper is published. Please apologise for any inconvenience to the researcher in the meantime."

The FFN202 is sold by Abcam as ab120867:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=120867).

Please let me know if you have any questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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