Key features and details
- Novel, pH responsive FFN. Enables pH measurement of secretory vesicles.
- CAS Number: 1234064-29-9
- Purity: > 97%
- Soluble in water to 50 mM
- Form / State: Solid
- Source: Synthetic
Product nameFFN202 (Mini 202), Fluorescent VMAT1 and VMAT2 substrate
DescriptionNovel, pH responsive FFN. Enables pH measurement of secretory vesicles.
Novel, pH responsive fluorescent false neurotransmitter (FFN). Rodent VMAT1 and VMAT2 substrate. Enables in situ pH measurement of secretory catecholamine vesicles in PC-12 cells. Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy and standard fluorescent microscopy. Compatible with GFP tags.
Chemical name3-(2-Aminoethyl)-6-chloro-7-hydroxy-2H-1-benzopyran-2-one 2,2,2-trifluoroacetate
Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 50 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120867 has been referenced in 4 publications.
- Ahmad MF et al. Perivascular Adipocytes Store Norepinephrine by Vesicular Transport. Arterioscler Thromb Vasc Biol 39:188-199 (2019). PubMed: 30567483
- Izumi H et al. Recycling endosomal CD133 functions as an inhibitor of autophagy at the pericentrosomal region. Sci Rep 9:2236 (2019). PubMed: 30783186
- Crummy E et al. The priming factor CAPS1 regulates dense-core vesicle acidification by interacting with rabconnectin3ß/WDR7 in neuroendocrine cells. J Biol Chem 294:9402-9415 (2019). PubMed: 31004036
- Wu Z et al. Nanodisc-cell fusion: control of fusion pore nucleation and lifetimes by SNARE protein transmembrane domains. Sci Rep 6:27287 (2016). PubMed: 27264104