Key features and details
- First novel fluorescent false neurotransmitter. Enables optical imaging of presynaptic terminal activity.
- CAS Number: 1004548-96-2
- Soluble in DMSO to 100 mM and in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
Product nameFFN511, Fluorescent substrate for VMAT2
DescriptionFirst novel fluorescent false neurotransmitter. Enables optical imaging of presynaptic terminal activity.
- Fluorescent False Neurotransmitter 511
First novel fluorescent false neurotransmitter. Enables optical imaging of presynaptic terminal activity.
Inhibits 5-HT binding to VMAT2 (IC50 = 1 µM (comparable to dopamine itself)). Released via exocytosis in the striatum in mouse acute slice preparation. Labels dopamine and other presynaptic terminals in the striatum.
Compatible with GFP tags and other optical probes. Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy.
View the technique online
For highly selective labelling of dopaminergic presynaptic termainals FFN102 (ab120866) is recommended.
Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM and in ethanol to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.To make a 10 μM solution for slice incubation, 25 μl of a 20 mM FFN511 stock solution in DMSO is added to 50 ml of ACSF. This results in a 10 μM FFN511 solution in ACSF containing 0.05% DMSO.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120331 has been referenced in 3 publications.
- Paul N et al. Activation of the glucocorticoid receptor rapidly triggers calcium-dependent serotonin release in vitro. CNS Neurosci Ther 27:753-764 (2021). PubMed: 33715314
- Huang M et al. Drug testing complementary metal-oxide-semiconductor chip reveals drug modulation of transmitter release for potential therapeutic applications. J Neurochem 151:38-49 (2019). PubMed: 31274190
- Shin W et al. Visualization of Membrane Pore in Live Cells Reveals a Dynamic-Pore Theory Governing Fusion and Endocytosis. Cell 173:934-945.e12 (2018). PubMed: 29606354