Overview

  • Product name

  • Description

    Rabbit polyclonal to FGD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FGD1 aa 50-100. The exact sequence is proprietary.
    Database link: P98174

  • Positive control

    • IP: HeLa cell lysate.
  • General notes

    ab226473 has not performed satisfactorily when used for WB of FGD1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of FGD1.

Properties

Applications

Our Abpromise guarantee covers the use of ab226473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.
  • Tissue specificity

    Expressed in fetal heart, brain, lung, kidney and placenta. Less expressed in liver; adult heart, brain, lung, pancreas and skeletal muscle.
  • Involvement in disease

    Aarskog-Scott syndrome
    Defects in FGD1 are found in a patient with non-syndromal X-linked mental retardation.
  • Sequence similarities

    Contains 1 DH (DBL-homology) domain.
    Contains 1 FYVE-type zinc finger.
    Contains 2 PH domains.
  • Domain

    The DH domain is involved in interaction with CCPG1.
  • Cellular localization

    Cytoplasm. Cell projection, lamellipodium. Cell projection, ruffle. Cytoplasm, cytoskeleton. Associated with membrane ruffles and lamellipodia.
  • Information by UniProt
  • Database links

  • Alternative names

    • AAS antibody
    • Faciogenital dysplasia 1 protein antibody
    • FGD1 antibody
    • FGD1_HUMAN antibody
    • FGDY antibody
    • FYVE RhoGEF and PH domain containing protein 1 antibody
    • FYVE, RhoGEF and PH domain-containing protein 1 antibody
    • Rho/Rac GEF antibody
    • Rho/Rac guanine nucleotide exchange factor FGD1 antibody
    • ZFYVE3 antibody
    • Zinc finger FYVE domain containing protein 3 antibody
    • Zinc finger FYVE domain-containing protein 3 antibody
    see all

Images

  • FGD1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN buffer; 1.0 mg for IP, 20% of IP loaded) with ab226473 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226473 at 1 µg/ml.

    Lane 1: ab226473 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 30 seconds.

References

ab226473 has not yet been referenced specifically in any publications.

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