Anti-FGD1 antibody - C-terminal (ab227302)

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Key features and details

  • Rabbit polyclonal to FGD1 - C-terminal
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-FGD1 antibody - C-terminal
    See all FGD1 primary antibodies

  • Description

    Rabbit polyclonal to FGD1 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Recombinant fragment within Human FGD1 (C terminal). The exact sequence is proprietary.
    Database link: P98174

  • Positive control

    • WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab227302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 106 kDa.

Target

  • Function

    Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.

  • Tissue specificity

    Expressed in fetal heart, brain, lung, kidney and placenta. Less expressed in liver; adult heart, brain, lung, pancreas and skeletal muscle.

  • Involvement in disease

    Aarskog-Scott syndrome
    Defects in FGD1 are found in a patient with non-syndromal X-linked mental retardation.

  • Sequence similarities

    Contains 1 DH (DBL-homology) domain.
    Contains 1 FYVE-type zinc finger.
    Contains 2 PH domains.

  • Domain

    The DH domain is involved in interaction with CCPG1.

  • Cellular localization

    Cytoplasm. Cell projection, lamellipodium. Cell projection, ruffle. Cytoplasm, cytoskeleton. Associated with membrane ruffles and lamellipodia.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AAS antibody
    • Faciogenital dysplasia 1 protein antibody
    • FGD1 antibody
    • FGD1_HUMAN antibody
    • FGDY antibody
    • FYVE RhoGEF and PH domain containing protein 1 antibody
    • FYVE, RhoGEF and PH domain-containing protein 1 antibody
    • Rho/Rac GEF antibody
    • Rho/Rac guanine nucleotide exchange factor FGD1 antibody
    • ZFYVE3 antibody
    • Zinc finger FYVE domain containing protein 3 antibody
    • Zinc finger FYVE domain-containing protein 3 antibody
    see all

Images

  • Western blot - Anti-FGD1 antibody - C-terminal (ab227302)
    Western blot - Anti-FGD1 antibody - C-terminal (ab227302)
    All lanes : Anti-FGD1 antibody - C-terminal (ab227302) at 1/500 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
    Lane 3 : IMR32 (human brain neuroblast cell line) whole cell extract
    Lane 4 : SK-N-AS (human brain neuroblastoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 106 kDa



    7.5% SDS-PAGE gel.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab227302? Please let us know so that we can cite the reference in this datasheet.

ab227302 has not yet been referenced specifically in any publications.

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