Anti-FGD1 antibody - C-terminal (ab227302)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-FGD1 antibody - C-terminal
See all FGD1 primary antibodies -
Description
Rabbit polyclonal to FGD1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment within Human FGD1 (C terminal). The exact sequence is proprietary.
Database link: P98174 -
Positive control
- WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab227302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/3000. Predicted molecular weight: 106 kDa. |
Target
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Function
Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape. -
Tissue specificity
Expressed in fetal heart, brain, lung, kidney and placenta. Less expressed in liver; adult heart, brain, lung, pancreas and skeletal muscle. -
Involvement in disease
Aarskog-Scott syndrome
Defects in FGD1 are found in a patient with non-syndromal X-linked mental retardation. -
Sequence similarities
Contains 1 DH (DBL-homology) domain.
Contains 1 FYVE-type zinc finger.
Contains 2 PH domains. -
Domain
The DH domain is involved in interaction with CCPG1. -
Cellular localization
Cytoplasm. Cell projection, lamellipodium. Cell projection, ruffle. Cytoplasm, cytoskeleton. Associated with membrane ruffles and lamellipodia. - Information by UniProt
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Database links
- Entrez Gene: 2245 Human
- Entrez Gene: 14163 Mouse
- Omim: 305400 Human
- SwissProt: P98174 Human
- SwissProt: P52734 Mouse
- Unigene: 631767 Human
- Unigene: 709201 Human
- Unigene: 475704 Mouse
see all -
Alternative names
- AAS antibody
- Faciogenital dysplasia 1 protein antibody
- FGD1 antibody
see all
Images
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All lanes : Anti-FGD1 antibody - C-terminal (ab227302) at 1/500 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 (human brain neuroblast cell line) whole cell extract
Lane 4 : SK-N-AS (human brain neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 106 kDa7.5% SDS-PAGE gel.
References
ab227302 has not yet been referenced specifically in any publications.