Recombinant
RabMAb

Anti-FGD3 antibody [EPR12448] (ab174857)

Overview

  • Product name
    Anti-FGD3 antibody [EPR12448]
  • Description
    Rabbit monoclonal [EPR12448] to FGD3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human FGD3 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q5JSP0

  • Positive control
    • 293T, K562, T.T and A549 cell lysates; A549 and K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12448
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 79 kDa.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Promotes the formation of filopodia. May activate CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.
    • Sequence similarities
      Contains 1 DH (DBL-homology) domain.
      Contains 1 FYVE-type zinc finger.
      Contains 2 PH domains.
    • Cellular localization
      Cytoplasm. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • Faciogenital dysplasia 3 antibody
      • FGD1 family, member 3 antibody
      • FGD3 antibody
      • FGD3_HUMAN antibody
      • FYVE antibody
      • FYVE, RhoGEF and PH domain containing 3 antibody
      • FYVE, RhoGEF and PH domain containing protein 3 antibody
      • RhoGEF and PH domain-containing protein 3 antibody
      • ZFYVE5 antibody
      • Zinc finger FYVE domain containing protein 5 antibody
      • Zinc finger FYVE domain-containing protein 5 antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized K562 cells labeling FGD3 using ab174857 at 1/10 dilution  (red)  or a rabbit IgG  (green).

    • Immunofluorescence analysis of A549 cells, labeling FGD3 using ab174857 at 1/50 dilution. DAPI nuclear staining (blue).

    • All lanes : Anti-FGD3 antibody [EPR12448] (ab174857) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : T.T cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 79 kDa

    References

    ab174857 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174857.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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