Product nameAnti-FGD3 antibody [EPR12448]
DescriptionRabbit monoclonal [EPR12448] to FGD3
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Orangutan
Synthetic peptide within Human FGD3 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: Q5JSP0
- 293T, K562, T.T and A549 cell lysates; A549 and K562 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 79 kDa.|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPromotes the formation of filopodia. May activate CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 FYVE-type zinc finger.
Contains 2 PH domains.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton.
- Information by UniProt
- Faciogenital dysplasia 3 antibody
- FGD1 family, member 3 antibody
- FGD3 antibody
Flow cytometric analysis of permeabilized K562 cells labeling FGD3 using ab174857 at 1/10 dilution (red) or a rabbit IgG (green).
Immunofluorescence analysis of A549 cells, labeling FGD3 using ab174857 at 1/50 dilution. DAPI nuclear staining (blue).
All lanes : Anti-FGD3 antibody [EPR12448] (ab174857) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : K562 cell lysate
Lane 3 : T.T cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 79 kDa
ab174857 has not yet been referenced specifically in any publications.