Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: PBS, pH 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab67681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 65 kDa.|
RelevanceFGD5 may activate CDC42, a member of the Ras like family of Rho and Rac proteins, by exchanging bound GDP for free GTP. It may also play a role in regulating the actin cytoskeleton and cell shape. FGD5 contains one DH (DBL-homology) domain one FYVE type zinc finger and two PH domains. There are two isoforms.
Cellular localizationCytoplasm, cytoskeleton
- FYVE, RhoGEF and PH domain containing protein 5 antibody
- ZFYVE23 antibody
- Zinc finger FYVE domain containing protein 23 antibody
All lanes : Anti-FGD5 antibody (ab67681) at 1/500 dilution
Lane 1 : FGD5 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Observed band size: 65 kDa why is the actual band size different from the predicted?
ab67681 has not yet been referenced specifically in any publications.