Overview

  • Product name

  • Description

    Goat polyclonal to FGE
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human FGE aa 314-327 (internal sequence).
    Sequence:

    C-ETLNPKGPPSGKDR


    Database link: NP_877437.2

  • Positive control

    • Mouse pancreas, Mouse eye and Human kidney lysates
  • General notes

     This product was previously labelled as SUMF1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab91479 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa).

Target

  • Function

    Using molecular oxygen and an unidentified reducing agent, oxidizes a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also called C(alpha)-formylglycine. Known substrates include GALNS, ARSA, STS and ARSE.
  • Tissue specificity

    Ubiquitous. Highly expressed in kidney, pancreas and liver. Detected at lower levels in leukocytes, lung, placenta, small intestine, skeletal muscle and heart.
  • Pathway

    Protein modification; sulfatase oxidation.
  • Involvement in disease

    Defects in SUMF1 are the cause of multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Inheritance is autosomal recessive.
  • Sequence similarities

    Belongs to the sulfatase-modifying factor family.
  • Post-translational
    modifications

    N-glycosylated. Contains high-mannose-type oligosaccharides.
  • Cellular localization

    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC150436 antibody
    • AAPA3037 antibody
    • C alpha formylglycine generating enzyme 1 antibody
    • C-alpha-formylglycine-generating enzyme 1 antibody
    • FGE antibody
    • FGly generating enzyme antibody
    • MGC131853 antibody
    • Sulfatase modifying factor 1 [Precursor] antibody
    • Sulfatase-modifying factor 1 antibody
    • SUMF1 antibody
    • SUMF1_HUMAN antibody
    • UNQ3037 antibody
    see all

Images

  • Anti-FGE antibody (ab91479) at 1 µg/ml + Mouse Pancreas lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 40 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab91479 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab91479.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up