Key features and details
- Goat polyclonal to FGE
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FGE antibody
See all FGE primary antibodies
DescriptionGoat polyclonal to FGE
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog
- Mouse pancreas, Mouse eye and Human kidney lysates
This product was previously labelled as SUMF1
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab91479 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab91479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa).
1 hour primary incubation is recommended for this product.
FunctionUsing molecular oxygen and an unidentified reducing agent, oxidizes a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also called C(alpha)-formylglycine. Known substrates include GALNS, ARSA, STS and ARSE.
Tissue specificityUbiquitous. Highly expressed in kidney, pancreas and liver. Detected at lower levels in leukocytes, lung, placenta, small intestine, skeletal muscle and heart.
PathwayProtein modification; sulfatase oxidation.
Involvement in diseaseDefects in SUMF1 are the cause of multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Inheritance is autosomal recessive.
Sequence similaritiesBelongs to the sulfatase-modifying factor family.
modificationsN-glycosylated. Contains high-mannose-type oligosaccharides.
Cellular localizationEndoplasmic reticulum lumen.
- Information by UniProt
- MGC150436 antibody
- AAPA3037 antibody
- C alpha formylglycine generating enzyme 1 antibody
Anti-FGE antibody (ab91479) at 1 µg/ml + Mouse Pancreas lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab91479 has not yet been referenced specifically in any publications.