Overview

  • Product name
    Anti-FGF basic antibody [MC-GF1]
    See all FGF2 primary antibodies
  • Description
    Mouse monoclonal [MC-GF1] to FGF basic
  • Host species
    Mouse
  • Specificity
    GF1 recognises basic fibroblast growth factor (bFGF).
  • Tested applications
    Suitable for: ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr 1/250. PubMed: 17327226Fix with acetone. Not suitable for detecting bFGF bound to cell surface receptors.

Target

  • Function
    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • Tissue specificity
    Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • Sequence similarities
    Belongs to the heparin-binding growth factors family.
  • Post-translational
    modifications
    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • Cellular localization
    Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic fibroblast growth factor antibody
    • Basic fibroblast growth factor bFGF antibody
    • BFGF antibody
    • FGF 2 antibody
    • FGF B antibody
    • FGF-2 antibody
    • Fgf2 antibody
    • FGF2 basic antibody
    • FGF2_HUMAN antibody
    • FGFB antibody
    • Fibroblast growth factor 2 (basic) antibody
    • Fibroblast growth factor 2 antibody
    • Fibroblast growth factor, basic antibody
    • HBGF 2 antibody
    • HBGF-2 antibody
    • HBGF2 antibody
    • HBGH 2 antibody
    • HBGH2 antibody
    • Heparin binding growth factor 2 precursor antibody
    • Heparin-binding growth factor 2 antibody
    • Prostatropin antibody
    see all

Images

  • ab181 staining FGF basic in normal (upper) and diabetic (lower) porcine aortic tissue by Immunohistochemistry (Frozen sections).

    Tissue was fixed with acetone and air dried for 24 hours. Sections were blocked for endogenous peroxidase activity and incubated with primary antibody at 1/250 dilution. Staining was detected using DAB and counterstained with hematoxylin.

References

This product has been referenced in:
  • Miao H  et al. Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor, and Pigment Epithelium-Derived Factor Expression in the Neovascular Iris in Retinal Diseases. J Ophthalmol 2018:8025951 (2018). Read more (PubMed: 29850214) »
  • Liu B  et al. Expressions of TGF-ß2, bFGF and ICAM-1 in lens epithelial cells of complicated cataract with silicone oil tamponade. Int J Ophthalmol 10:1034-1039 (2017). Read more (PubMed: 28730103) »
See all 7 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - whole (Stomach)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
30 µg
Specification
Stomach
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 28 2018

Application
Flow Cytometry
Sample
Mouse Cell (Primary hepatocytes)
Permeabilization
Yes - Fluidigm Fix and Perm Buffer
Gating Strategy
Positive cells for APC
Specification
Primary hepatocytes
Preparation
Cell harvesting/tissue preparation method: Liver dissection, followed by mashing the liver to obtain hepatocytes
Sample buffer: PBS + 10% Bovine Serum
Fixation
Formaaldehyde

Abcam user community

Verified customer

Submitted May 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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