Key features and details
- Mouse polyclonal to FGF1 alpha
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FGF1 alpha antibody
DescriptionMouse polyclonal to FGF1 alpha
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Chicken, Hamster, Cow, Pig, Orangutan
Recombinant full length protein corresponding to Human FGF1 alpha aa 1-155.
Database link: NP_000791.1
- FGF1 alpha transfected 293T cell lysate; HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Constituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab169748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
Cellular localizationSecreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
- Information by UniProt
- Acidic fibroblast growth factor antibody
- aFGF antibody
- Beta-endothelial cell growth factor antibody
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling FGF1 alpha with ab169748 at 10µg/ml.
All lanes : Anti-FGF1 alpha antibody (ab169748) at 1 µg/ml
Lane 1 : FGF1 alpha transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 17 kDa
ab169748 has been referenced in 2 publications.
- Ichikawa K et al. Activated FGF2 signaling pathway in tumor vasculature is essential for acquired resistance to anti-VEGF therapy. Sci Rep 10:2939 (2020). PubMed: 32076044
- Han J et al. Acid fibroblast growth factor facilitates the progression of atherosclerotic plaques regardless of alterations in serum lipid expression levels in HFD-fed ApoE-/- mice. Mol Med Rep 18:1025-1030 (2018). PubMed: 29845277