Product nameAnti-FGF1 antibody
See all FGF1 primary antibodies
DescriptionRabbit polyclonal to FGF1
Tested applicationsSuitable for: WB, ELISA, Neutralising, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Highly pure (>98%) recombinant FGF1 (human Fibroblast Growth Factor-acidic).
- Purchase matching WB positive control:Recombinant human FGF1 protein
- ICC/IF: HepG2 cells.
FormLyophilised:Reconstitute with 200µl of sterile water.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPBS, pH 7.4, no preservative, sterile filtered
Concentration information loading...
PurityImmunogen affinity purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab9588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|ELISA||Use a concentration of 0.5 µg/ml.|
|Neutralising||Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hFGF1 (30.0 ng/ml), a concentration of 0.46 - 0.9 µg/ml of this antibody is required.|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
Cellular localizationSecreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
- Information by UniProt
- Acidic fibroblast growth factor antibody
- aFGF antibody
- Beta endothelial cell growth factor antibody
ICC/IF image of ab9588 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9588, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab9588 staining FGF1 in rat dorsal root ganglia tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in paraformaldehyde and permeabilized using 0.25% Triton X-100. Samples were then blocked 0.25% BSA for 1 hour at 25°C, then incubated with ab9588 at a 1/200 dilution for 16 hours at 4°C. The secondary used was a donkey anti-rabbit polyclonal conjugated to rhodamine, 1/500 dilution.
This product has been referenced in:
- Liu X et al. Long non-coding RNA Gas5 regulates proliferation and apoptosis in HCS-2/8 cells and growth plate chondrocytes by controlling FGF1 expression via miR-21 regulation. J Biomed Sci 25:18 (2018). Read more (PubMed: 29490650) »
- Li M et al. FGF1 Mediates Overnutrition-Induced Compensatory ß-Cell Differentiation. Diabetes 65:96-109 (2016). IF ; Zebrafish . Read more (PubMed: 26420862) »