Overview

  • Product name

    Anti-FGF1 antibody [EPR19989]
    See all FGF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19989] to FGF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein within Human FGF1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P05230

  • Positive control

    • WB: Human fetal kidney, fetal brain and glioma lysates; U-87 MG and Neuro-2a whole cell lysates; Mouse spinal cord, heart and kidney lysates; Rat heart lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab207321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 16,18 kDa (predicted molecular weight: 17 kDa).

Target

  • Function

    The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
  • Sequence similarities

    Belongs to the heparin-binding growth factors family.
  • Cellular localization

    Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acidic fibroblast growth factor antibody
    • aFGF antibody
    • Beta endothelial cell growth factor antibody
    • Beta-endothelial cell growth factor antibody
    • ECGF antibody
    • ECGF beta antibody
    • ECGF-beta antibody
    • ECGFA antibody
    • ECGFB antibody
    • Endothelial Cell Growth Factor alpha antibody
    • Endothelial Cell Growth Factor beta antibody
    • FGF 1 antibody
    • FGF alpha antibody
    • Fgf1 antibody
    • FGF1_HUMAN antibody
    • FGFA antibody
    • Fibroblast Growth Factor 1 Acidic antibody
    • Fibroblast growth factor 1 antibody
    • GLIO703 antibody
    • HBGF 1 antibody
    • HBGF-1 antibody
    • HBGF1 antibody
    • Heparin binding growth factor 1 antibody
    • Heparin binding growth factor 1 precursor antibody
    • Heparin-binding growth factor 1 antibody
    see all

Images

  • All lanes : Anti-FGF1 antibody [EPR19989] (ab207321) at 1/1000 dilution

    Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 17 kDa
    Observed band size: 16,18 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 1 second; Lane 2/4: 5 seconds; Lane 3: 3 seconds.

    The observed molecular weight is consistent with the literature (PMID: 16091423).

  • All lanes : Anti-FGF1 antibody [EPR19989] (ab207321) at 1/1000 dilution

    Lane 1 : Human glioma lysate
    Lane 2 : Mouse spinal cord lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : Mouse kidney lysate
    Lane 5 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
    Lanes 2-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 17 kDa
    Observed band size: 16,18 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1/2/5: 3 minutes; Lane 3: 10 seconds; Lane 4: 15 seconds.

    The observed molecular weight is consistent with the literature (PMID: 16091423).

References

This product has been referenced in:

  • Liang G  et al. Fibroblast growth factor 1 ameliorates diabetic nephropathy by an anti-inflammatory mechanism. Kidney Int 93:95-109 (2018). IHC-P, IHC-Fr . Read more (PubMed: 28750927) »
See 1 Publication for this product

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