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We are interested in quantifying FGF10 concentration using ELISA. We saw the result of ELISA on your home page (https://www.abcam.co.jp/FGF10-antibody-ab90207.html#FGF10-Primary-antibodies-ab90207-3.jpg). Will you please provide us the detailed protocol of this procedure?
Asked on May 08 2012
Thank you for your enquiry.
The data on the datasheet is a sandwich ELISA result. This ab90207 FGF10 antibody can be paired with ab83439 FGF10 antibody (Biotin), where ab90207 is the capture antibody and ab83439 is the detection antibody.
1) ab90207 use at 5ug/ml
2) ab83439 use at 0.25 - 1 µg/ml
For more protocol details, please refer to our protocol links below.
I hope this information will be helpful. If there is anything else that I can help you with please do not hesitate to contact me.
Have a nice day!
Answered on May 08 2012