Question (46409) | Biotin Anti-FGF10 antibody (ab83439)

Go to datasheet (ab83439)

Question

We are interested in quantifying FGF10 concentration using ELISA. We saw the result of ELISA on your home page (https://www.abcam.co.jp/FGF10-antibody-ab90207.html#FGF10-Primary-antibodies-ab90207-3.jpg). Will you please provide us the detailed protocol of this procedure?

Answer

Thank you for your enquiry.

The data on the datasheet is a sandwich ELISA result. This ab90207 FGF10 antibody can be paired with ab83439 FGF10 antibody (Biotin), where ab90207 is the capture antibody and ab83439 is the detection antibody.

Recommended dilutions:
1) ab90207 use at 5ug/ml
2) ab83439 use at 0.25 - 1 µg/ml

For more protocol details, please refer to our protocol links below.

https://www.abcam.com/index.html?pageconfig=resource&rid=11422 (English)
https://www.abcam.co.jp/index.html?pageconfig=resource&rid=11422 (Japanese)
https://www.abcam.com/ps/pdf/protocols/Sandwich_Elisa_diagram.pdf (Diagram)

I hope this information will be helpful. If there is anything else that I can help you with please do not hesitate to contact me.

Have a nice day!