Overview

  • Product name

  • Description

    Rabbit polyclonal to FGF12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Sheep, Chicken, Cow, Human, Xenopus laevis, Zebrafish
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1-168 of Human FGF12. (Uniprot: P61328)

  • Positive control

    • Mouse brain lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 27 kDa.

Target

  • Function

    Probably involved in nervous system development and function.
  • Tissue specificity

    Brain, eye and testis; highly expressed in embryonic retina, olfactory epithelium, olfactory bulb, and in a segmental pattern of the body wall; in adult olfactory bulb, less in cerebellum, deep cerebellar nuclei, cortex and multiple midbrain structures.
  • Sequence similarities

    Belongs to the heparin-binding growth factors family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • EIEE47 antibody
    • FGF-12 antibody
    • Fgf12 antibody
    • FGF12_HUMAN antibody
    • FGF12B antibody
    • FHF-1 antibody
    • FHF1 antibody
    • Fibroblast growth factor 12 antibody
    • Fibroblast growth factor 12B antibody
    • Fibroblast growth factor FGF 12b antibody
    • Fibroblast growth factor homologous factor 1 antibody
    • Myocyte activating factor antibody
    • Myocyte-activating factor antibody
    see all

Images

  • Anti-FGF12 antibody (ab125682) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 27 kDa

References

ab125682 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question

Dear Sir or Madam,

a) we've ordered and used the ab58157 for immunofluorescence analyses in the brain (reordered now at least 3 times). At the beginning (2009), this antibody (as we can recollect) was not designated as "reacts with human" only but I also designated to theoretically cross-react with mouse! This also makes sense as the AA sequences are identical between these two species: Please compare, NP_066360.1 and NP_898887.1. Why now was there a change implemented on your website with regards to specificity? If the antibody does not recognize the same AA sequence protein in mouse any more, do you have explanation for this discrepancy?Partly (or presumably) as a consequence from it labelled as human specific, we now have response from reviewers who seriously doubt the specificity of this antibody).

b) The reviewers request the use of a blocking peptide, - would this be available, please, and what is the nature of the immunogen, really the whole length protein for a monoclonal antibody? (https://www.abcam.com/FGF12-antibody-ab58157.html).

c) However, we've had recently some problems reproducing earlier results in mouse tissue, and getting a band in western blot using the antibody with this very same order number. Here is another fact that startles me. For the Western blot, you now have demonstrated a band at 47.5 kDa now, using this antibody, but before it was around 27kDa (in human), and that is what we had earlier using this antibody in mouse tissue (e.g.: see attached western blot from the 2009 lots), and is only shown in the newer released antibody (ab125682), a predicted band size around 27kDa, while next to it you show a Western blot showing around 20 kDa?

I hope you could clarify a few of the above points for us and provide us with a suggestion regarding the blocking peptide.

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Answer

Regarding your enquiry, we have been informed that unfortunately there is no blocking peptide available for ab58157, clone 1D9.
The recombinant protein used to generate the antibody is FGF12 (Human) Recombinant Protein (aa 1-181) with a tag at the N-terminal. This protein is not in our catalog but it is commercially available. Please contact us if you want to order it and we will add it to our catalog.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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