Anti-FGF13 antibody [N235/22] (ab186300)
Key features and details
- Mouse monoclonal [N235/22] to FGF13
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-FGF13 antibody [N235/22] -
Description
Mouse monoclonal [N235/22] to FGF13 -
Host species
Mouse -
Specificity
ab186300 will not recognize isoforms 2, 3, 4 or 5 of human FGF13. It cross-reacts with FGF12 and FGF14. It has 100% identity with human, mouse and rat FGF12; 94% identity with human, mouse and rat FGF14; 81% identity to human and mouse FGF11. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Xenopus laevis, Orangutan -
Immunogen
Synthetic peptide corresponding to Human FGF13 aa 1-100 (N terminal).
Database link: Q92913 -
Positive control
- WB: Rat brain lysate. ICC/IF: SK-N-BE cells.
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General notes
1μg/mL of ab186300 was sufficient for detection of FGF13 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
The clone number has been updated from S235-22 to N235/22, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N235/22 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 28 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000. Predicted molecular weight: 28 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Probably involved in nervous system development and function. -
Tissue specificity
Nervous system. -
Sequence similarities
Belongs to the heparin-binding growth factors family. - Information by UniProt
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Database links
- Entrez Gene: 2258 Human
- Entrez Gene: 84488 Rat
- Omim: 300070 Human
- SwissProt: Q92913 Human
- SwissProt: Q9ERW3 Rat
- Unigene: 6540 Human
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Alternative names
- FGF 13 antibody
- FGF 2 antibody
- FGF-13 antibody
see all
Images
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SK-N-BE (Human neuroblastoma cell line) cells stained for FGF13 using ab186300 at a dilution of 1/100 (1 hour at RT) in ICC/IF.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 1 hour at RT.
Cells were fixed with 4% formaldehyde for 15 minutes at RT.
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Anti-FGF13 antibody [N235/22] (ab186300) at 1/200 dilution + Rat brain membrane lysate at 15 µg
Secondary
Goat Anti-Mouse IgG: HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 28 kDa
Exposure time: 6 minutesBlock: 2% BSA and 2% skimmed milk in 1X TBST.
Incubated with primary antibody for 16 hours at 4°C.
Incubated with secondary antibody for 1 hour at RT.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab186300 has been referenced in 1 publication.
- Wu Y et al. lncRNA LINC00963 downregulation regulates colorectal cancer tumorigenesis and progression via the miR-10b/FGF13 axis. Mol Med Rep 23:N/A (2021). PubMed: 33495804