Overview

  • Product name

    Anti-FGF13 antibody [N235/22]
    See all FGF13 primary antibodies
  • Description

    Mouse monoclonal [N235/22] to FGF13
  • Host species

    Mouse
  • Specificity

    ab186300 will not recognize isoforms 2, 3, 4 or 5 of human FGF13. It cross-reacts with FGF12 and FGF14. It has 100% identity with human, mouse and rat FGF12; 94% identity with human, mouse and rat FGF14; 81% identity to human and mouse FGF11.
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Xenopus laevis, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human FGF13 aa 2-18 (N terminal).
    Sequence:

    AAAIASSLIRQKRQARE


    Database link: Q92913

  • Positive control

    • WB: Rat brain lysate. ICC/IF: SK-N-BE cells.
  • General notes

    1μg/mL of ab186300 was sufficient for detection of FGF13 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    The clone number has been updated from S235-22 to N235/22, both clone numbers name the same antibody clone.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N235/22
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 28 kDa.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

Images

  • SK-N-BE (Human neuroblastoma cell line) cells stained for FGF13 using ab186300 at a dilution of 1/100 (1 hour at RT) in ICC/IF.

    Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 1 hour at RT.

    Cells were fixed with 4% formaldehyde for 15 minutes at RT.

  • Anti-FGF13 antibody [N235/22] (ab186300) at 1/200 dilution + Rat brain membrane lysate at 15 µg

    Secondary
    Goat Anti-Mouse IgG: HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 28 kDa


    Exposure time: 6 minutes


     Block: 2% BSA and 2% skimmed milk in 1X TBST.

    Incubated with primary antibody for 16 hours at 4°C.

    Incubated with secondary antibody for 1 hour at RT.

References

ab186300 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab186300.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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