Product nameAnti-FGF13 antibody [N235/22]
See all FGF13 primary antibodies
DescriptionMouse monoclonal [N235/22] to FGF13
Specificityab186300 will not recognize isoforms 2, 3, 4 or 5 of human FGF13. It cross-reacts with FGF12 and FGF14. It has 100% identity with human, mouse and rat FGF12; 94% identity with human, mouse and rat FGF14; 81% identity to human and mouse FGF11.
Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Xenopus laevis, Orangutan
- WB: Rat brain lysate. ICC/IF: SK-N-BE cells.
1μg/mL of ab186300 was sufficient for detection of FGF13 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
The clone number has been updated from S235-22 to N235/22, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab186300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 28 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionProbably involved in nervous system development and function.
Tissue specificityNervous system.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
- Information by UniProt
- FGF 13 antibody
- FGF 2 antibody
- FGF-13 antibody
SK-N-BE (Human neuroblastoma cell line) cells stained for FGF13 using ab186300 at a dilution of 1/100 (1 hour at RT) in ICC/IF.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 1 hour at RT.
Cells were fixed with 4% formaldehyde for 15 minutes at RT.
Anti-FGF13 antibody [N235/22] (ab186300) at 1/200 dilution + Rat brain membrane lysate at 15 µg
Goat Anti-Mouse IgG: HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 28 kDa
Exposure time: 6 minutes
Block: 2% BSA and 2% skimmed milk in 1X TBST.
Incubated with primary antibody for 16 hours at 4°C.
Incubated with secondary antibody for 1 hour at RT.
ab186300 has not yet been referenced specifically in any publications.