Anti-FGF14 antibody (ab229610)
Key features and details
- Rabbit polyclonal to FGF14
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-FGF14 antibody
See all FGF14 primary antibodies -
Description
Rabbit polyclonal to FGF14 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow -
Positive control
- WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. ICC/IF: U-87 MG cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 28 kDa.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 28 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
-
Relevance
FGF14 is probably involved in nervous system development and function. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 395554 Chicken
- Entrez Gene: 615488 Cow
- Entrez Gene: 2259 Human
- Entrez Gene: 14169 Mouse
- Entrez Gene: 63851 Rat
- Omim: 601515 Human
- SwissProt: Q92915 Human
- SwissProt: P70379 Mouse
see all -
Alternative names
- bA397O8.2 antibody
- FGF 14 antibody
- FHF 4 antibody
see all
Images
-
All lanes : Anti-FGF14 antibody (ab229610) at 1/500 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 (Human brain neuroblast cell line) whole cell extract
Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 28 kDa12% SDS-PAGE gel.
-
4% paraformaldehyde-fixed U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for FGF14 (green) using ab229610 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab229610 has been referenced in 1 publication.
- Turkowski K et al. Fibroblast Growth Factor-14 Acts as Tumor Suppressor in Lung Adenocarcinomas. Cells 9:N/A (2020). PubMed: 32707902