Anti-FGF17 antibody (ab9990)

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Sandwich ELISA - Anti-FGF17 antibody (ab9990)

    Key features and details

    • Rabbit polyclonal to FGF17
    • Suitable for: Sandwich ELISA
    • Isotype: unknown

    Overview

    • Product name

      Anti-FGF17 antibody
      See all FGF17 primary antibodies

    • Description

      Rabbit polyclonal to FGF17

    • Host species

      Rabbit

    • Tested Applications & Species

      Application Species
      sELISA
      Recombinant fragment
      See all applications and species data

    • Immunogen

      Highly pure (>98%) recombinant hFGF-17 (human Fibroblast Growth Factor-17)

    • Positive control

      • Recombinant human FGF17 protein (ab9991) can be used as a positive control in WB.

    Properties

    • Form

      Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

    • Storage buffer

      No preservative, sterile filtered
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      unknown

    • Light chain type

      unknown

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab9990 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    sELISA
    Recombinant fragment
    Application Abreviews Notes
    Sandwich ELISA
    Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [MM0284-5H27] to FGF17 (ab89270).

    For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab89270 as Capture.
    Notes
    Sandwich ELISA
    Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [MM0284-5H27] to FGF17 (ab89270).

    For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab89270 as Capture.

    Target

    • Function

      Plays an important role in the regulation of embryonic development and as signaling molecule in the induction and patterning of the embryonic brain. Required for normal brain development.

    • Tissue specificity

      Preferentially expressed in the embryonic brain.

    • Sequence similarities

      Belongs to the heparin-binding growth factors family.

    • Developmental stage

      Detected in embryos at E14.5, but not at E10.5 and E19.5. Preferentially expressed in the neuroepithelia of the isthmus and septum of the embryonic brain at E14.5.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Alternative names

      • FGF 13 antibody
      • FGF 17 antibody
      • FGF-17 antibody
      • FGF13 antibody
      • Fgf17 antibody
      • FGF17_HUMAN antibody
      • Fibroblast growth factor 17 antibody
      • HH20 antibody
      see all

    Images

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab9990? Please let us know so that we can cite the reference in this datasheet.

    ab9990 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    Hello, I was wondering if you could tell me smallest amount of FGF-17 detectable in a western blot using Ab9990 for detection? Thank You!

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    Answer

    Thank yoiu for your enquiry. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-17 using ab9990 FGF17 antibody is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Some lots may be more sensitive than this. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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    Question

    Thank you for your quick reply! Since I am planning on trying the antibody on chicken or mouse proteins, and we do not know whether it would recognize them, I wonder whether I could ask for a sample size to try or to purchase. Thanks, and have a nice day!

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    Answer

    Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abpoints which can be redeemed on a number of rewards (a further 100 Abpoints will be offered for an image). Please contact us again if you have any additional questions, and happy holidays.

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    Question

    I wonder whether this anti-Fgf17 antibody may cross react with Fgf17 in chicken and mice. In addition, could you provide any references when people tried to use it for Western blot?

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    Answer

    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with chicken and mouse have not yet been tested for use with ab9990. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 points will be offered for an image). Presently, we're not aware of any publications which feature the use of this antibody. Please rest assured that our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Please contact us again if you have any additional questions.

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