Overview

  • Product name

  • Description

    Rabbit polyclonal to FGF19
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human FGF19 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab93789)

  • Positive control

    • ICC/IF: HeLa cells. WB: Recombinant Human FGF19 protein (ab73213), Human liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 28906590

Target

  • Function

    Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB.
  • Tissue specificity

    Expressed in fetal brain, cartilage, retina, and adult gall bladder.
  • Sequence similarities

    Belongs to the heparin-binding growth factors family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • FGF 19 antibody
    • FGF-19 antibody
    • FGF15 antibody
    • FGF19 antibody
    • FGF19_HUMAN antibody
    • Fibroblast growth factor 15 antibody
    • Fibroblast growth factor 19 antibody
    see all

Images

  • Anti-FGF19 antibody (ab85042) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 24 kDa
    Additional bands at: 19 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes
  • ICC/IF image of ab85042 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85042, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Ipharraguerre IR  et al. Antimicrobial promotion of pig growth is associated with tissue-specific remodeling of bile acid signature and signaling. Sci Rep 8:13671 (2018). Read more (PubMed: 30209339) »
  • Zhang X  et al. FGF19 genetic amplification as a potential therapeutic target in lung squamous cell carcinomas. Thorac Cancer 8:655-665 (2017). IHC-P . Read more (PubMed: 28906590) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for your reply. No, the discount code does not need to be mentioned when placing the first order. Once your Abreview is submitted, the discount code can be redeemed on a future order for a free primary antibody of your choice.

Read More
Abreviews
Application
Western blot
Sample
Pig Tissue lysate - whole (Ileum)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
60 µg
Specification
Ileum
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 25 2016

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Pig Cell lysate - whole cell (Porcine FGF19 protein overexpressed in HEK293 cell)
Specification
Porcine FGF19 protein overexpressed in HEK293 cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Mar 24 2015

Answer

DISCOUNT CODE: ***
Expiration date: December 7th, 2012

I am very pleased to hear you would like to accept our offer and test ab85042 in chicken. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for chicken and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question
Answer

Thank you for contacting us. Of the FGF19 antibodies that are currently in our catalog, I would recommend ab85042, which is 87% homologous with the chicken (NP_990005.1).

To our knowledge, ab85042 has not been tested in chicken. Therefore, I can offer a discount off a future purchase if you buy ab85042 now, test it in chicken and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab85042 in chicken. I will then send a discount code. This code must be issued before purchasing ab85042 so please wait for my reply before ordering.

2. Purchase ab85042 either by phone, fax, or online (www.abcam.com).

3. Test it in chicken.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab85042 turns out to be unsuitable for chicken, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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