Anti-FGF2 antibody (ab8880)

Rabbit polyclonal FGF2 antibody. Validated in IHC, ICC/IF and tested in Mouse, Human. Cited in 18 publication(s). Independently reviewed in 4 review(s). Immunogen corresponding to synthetic peptide.

Overview

  • Product name

  • Description

    Rabbit polyclonal to FGF2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Mammals
  • Immunogen

    Synthetic peptide within Cow FGF2 aa 1-23. The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HepG2 cells. IHC-Fr: Mouse brain and olfactory epithelium tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab8880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 21187124
ICC/IF 1/200.

Target

  • Function

    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • Tissue specificity

    Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • Sequence similarities

    Belongs to the heparin-binding growth factors family.
  • Post-translational
    modifications

    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • Cellular localization

    Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt
  • Database links

  • Alternative names

    • Basic fibroblast growth factor antibody
    • Basic fibroblast growth factor bFGF antibody
    • BFGF antibody
    • FGF 2 antibody
    • FGF B antibody
    • FGF-2 antibody
    • Fgf2 antibody
    • FGF2 basic antibody
    • FGF2_HUMAN antibody
    • FGFB antibody
    • Fibroblast growth factor 2 (basic) antibody
    • Fibroblast growth factor 2 antibody
    • Fibroblast growth factor, basic antibody
    • HBGF 2 antibody
    • HBGF-2 antibody
    • HBGF2 antibody
    • HBGH 2 antibody
    • HBGH2 antibody
    • Heparin binding growth factor 2 precursor antibody
    • Heparin-binding growth factor 2 antibody
    • Prostatropin antibody
    see all

Images

  • ICC/IF image of ab8880 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8880, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of mouse brain tissue (Frozen sections) labelling FGF2 with ab8880 at a dilution of 1/500. Tissue sections were fixed with Paraformaldehyde and permeabilized with 0.3 % Troton X-100. The secondary antibody used was Alexa Fluor® 594 conjugated goat polyclonal secondary at 1/400. 

    See Abreview

  • ab8880 staining FGF basic in Mouse olfactory epithelium tissue by Immunohistochemistry (Frozen sections). The sections were PFA-fixed prior to blocking with 1% blocking reagent from a tyramide signal amplification kit for 30 minutes at 23°C. The primary antibody was diluted 1/200 in the blocking reagent and incubated with the sample for 2 hours at 23°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/100.
    After secondary antibody incubation, the signal was amplified using a tyramide signal amplication kit with Alexa Fluor® 488 tyramide.

    See Abreview

References

This product has been referenced in:

  • Fu R  et al. A ZEB1/p53 signaling axis in stromal fibroblasts promotes mammary epithelial tumours. Nat Commun 10:3210 (2019). Read more (PubMed: 31324807) »
  • Wu X  et al. Downregulation of Linc-RNA activator of myogenesis LncRNA participates in FGF2 mediated-proliferation of human periodontal ligament stem cells. J Periodontol N/A:N/A (2019). Read more (PubMed: 31378921) »
See all 20 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA high pH
Permeabilization
Yes - Tween 20
Specification
Brain
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 30 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kiney)
Specification
Kiney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 14 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3 % Troton X 100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 22 2016

Abreviews
Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney cell line(COS7))
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
30 µg
Specification
Kidney cell line(COS7)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 28 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (olfactory epithelium)
Specification
olfactory epithelium
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.02% triton X-100
Blocking step
blocking reagent from tyramide signal amplification kit (Invitrogen) as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Apr 29 2011

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