For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    fgf2-antibody-ep1735-ab92337.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Angiogenesis Growth Factors FGF FGF
Share by email
RecombinantRabMAb

Recombinant Anti-FGF2 antibody [EP1735] (ab92337)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (5)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)
  • Immunoprecipitation - Anti-FGF2 antibody [EP1735] (ab92337)
  • Western blot - Anti-FGF2 antibody [EP1735] (ab92337)
  • Western blot - Anti-FGF2 antibody [EP1735] (ab92337)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)
  • Sandwich ELISA - Anti-FGF2 antibody [EP1735] (ab92337)
  • Anti-FGF2 antibody [EP1735] (ab92337)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1735] to FGF2
  • Suitable for: WB, IP, IHC-P, Sandwich ELISA
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
Product image
Alexa Fluor® 647 Anti-STAT5a antibody [E289] (ab194309)
Primary
Product image
Anti-LC3B antibody [EPR18709] - BSA and Azide free (ab221794)

View more associated products

Overview

  • Product name

    Anti-FGF2 antibody [EP1735]
    See all FGF2 primary antibodies
  • Description

    Rabbit monoclonal [EP1735] to FGF2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FGF2 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: K-562, and HeLa lysates. IHC-P: human breast, prostate and cerebrum tissue. IP: HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1735
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • FGF
    • FGF
    • Signal Transduction
    • Growth Factors/Hormones
    • FGF
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Neural Stem Cells
    • Extracellular
    • Cancer
    • Growth factors
    • FGF
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

Associated products

  • Alternative Versions

    • Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human FGF2 protein (Active) (ab9596)
  • Related Products

    • Recombinant human FGF2 protein (Active) (ab9596)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IP
1/10 - 1/100.
IHC-P (1)
1/2400.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [AS24] to FGF basic (ab17279) as Capture.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IP
1/10 - 1/100.
IHC-P
1/2400.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [AS24] to FGF basic (ab17279) as Capture.

Target

  • Function

    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • Tissue specificity

    Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • Sequence similarities

    Belongs to the heparin-binding growth factors family.
  • Post-translational
    modifications

    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • Cellular localization

    Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Target information above from: UniProt accession P09038 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2247 Human
    • Omim: 134920 Human
    • SwissProt: P09038 Human
    • Unigene: 284244 Human
    • Alternative names

      • Basic fibroblast growth factor antibody
      • Basic fibroblast growth factor bFGF antibody
      • BFGF antibody
      • FGF 2 antibody
      • FGF B antibody
      • FGF-2 antibody
      • Fgf2 antibody
      • FGF2 basic antibody
      • FGF2_HUMAN antibody
      • FGFB antibody
      • Fibroblast growth factor 2 (basic) antibody
      • Fibroblast growth factor 2 antibody
      • Fibroblast growth factor, basic antibody
      • HBGF 2 antibody
      • HBGF-2 antibody
      • HBGF2 antibody
      • HBGH 2 antibody
      • HBGH2 antibody
      • Heparin binding growth factor 2 precursor antibody
      • Heparin-binding growth factor 2 antibody
      • Prostatropin antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling FGF2 with Purified ab92337 at 1:2400 dilution (0.51 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunoprecipitation - Anti-FGF2 antibody [EP1735] (ab92337)
      Immunoprecipitation - Anti-FGF2 antibody [EP1735] (ab92337)
      ab92337 (Purified) at 1:70 dilution (2 µg) immunoprecipitating FGF2 in HeLa whole cell lysate.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
      Lane 2 (+): ab92337 & HeLa whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92337 in HeLa whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.
    • Western blot - Anti-FGF2 antibody [EP1735] (ab92337)
      Western blot - Anti-FGF2 antibody [EP1735] (ab92337)
      Anti-FGF2 antibody [EP1735] (ab92337) at 1/5000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 31 kDa
      Observed band size: 17-30 kDa why is the actual band size different from the predicted?



      ab92337 recoginzes 3 isoforms of FGF2

    • Western blot - Anti-FGF2 antibody [EP1735] (ab92337)
      Western blot - Anti-FGF2 antibody [EP1735] (ab92337)
      Anti-FGF2 antibody [EP1735] (ab92337) at 1/1000 dilution (Purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 31 kDa
      Observed band size: 17-30 kDa why is the actual band size different from the predicted?



      ab92337 recoginzes 3 isoforms of FGF2

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue sections labeling FGF2 with purified ab92337 at 1/1000 dilution (1.3 µg/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

      Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling FGF2 with purified ab92337 at 1/1000 dilution (1.3 µg/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

      Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Sandwich ELISA - Anti-FGF2 antibody [EP1735] (ab92337)
      Sandwich ELISA - Anti-FGF2 antibody [EP1735] (ab92337)

      Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (ab9596)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1µg/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (ab92337) at 0.5µg/ml.

    • Anti-FGF2 antibody [EP1735] (ab92337)
      Anti-FGF2 antibody [EP1735] (ab92337)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Western blot protocols
    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab92337? Please let us know so that we can cite the reference in this datasheet.

    ab92337 has been referenced in 5 publications.

    • Wei F  et al. Plasma endothelial cells-derived extracellular vesicles promote wound healing in diabetes through YAP and the PI3K/Akt/mTOR pathway. Aging (Albany NY) 12:12002-12018 (2020). PubMed: 32570219
    • Jia ZY  et al. The correlation between ultrasonographic features, bFGF, and the local invasiveness of thyroid papillary carcinoma. Medicine (Baltimore) 99:e20644 (2020). PubMed: 32590739
    • Zhu W  et al. Knockout of EGFL6 by CRISPR/Cas9 Mediated Inhibition of Tumor Angiogenesis in Ovarian Cancer. Front Oncol 10:1451 (2020). PubMed: 32983976
    • Zhu P  et al. Host Cellular Protein TRAPPC6A? Interacts with Influenza A Virus M2 Protein and Regulates Viral Propagation by Modulating M2 Trafficking. J Virol 91:N/A (2017). PubMed: 27795429
    • Xiao B  et al. TTF1, in the Form of Nanoparticles, Inhibits Angiogenesis, Cell Migration and Cell Invasion In Vitro and In Vivo in Human Hepatoma through STAT3 Regulation. Molecules 21:N/A (2016). PubMed: 27834930

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FGF2 antibody [EP1735]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Flex TRS High pH
    Specification
    Kidney
    Blocking step
    (agent) for 5 minute(s) · Concentration: 0.25% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Mr. Mark Butters

    Verified customer

    Submitted Feb 02 2021

    Western blot abreview for Anti-FGF basic antibody [EP1735]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (HepG2)
    Specification
    HepG2
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 19 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.