Recombinant Anti-FGF2 antibody [EP1735] (ab92337)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)](https://www.abcam.com/ps/products/92/ab92337/Images/ab92337-9-anti-fgf2-antibody-ep1735-immunohistochemistry-breast-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1735] to FGF2
- Suitable for: WB, IP, IHC-P, Sandwich ELISA
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FGF2 antibody [EP1735]
See all FGF2 primary antibodies -
Description
Rabbit monoclonal [EP1735] to FGF2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human FGF2 aa 100-200. The exact sequence is proprietary.
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Positive control
- WB: K-562, and HeLa lysates. IHC-P: human breast, prostate and cerebrum tissue. IP: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1735 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
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| IP |
1/10 - 1/100.
|
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| IHC-P | (1) |
1/2400.
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). |
| Sandwich ELISA |
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [AS24] to FGF basic (ab17279) as Capture.
|
| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa. |
|
IP
1/10 - 1/100. |
|
IHC-P
1/2400. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). |
|
Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [AS24] to FGF basic (ab17279) as Capture. |
Target
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Function
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107). -
Tissue specificity
Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Post-translational
modificationsPhosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. -
Cellular localization
Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. - Information by UniProt
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Database links
- Entrez Gene: 2247 Human
- Omim: 134920 Human
- SwissProt: P09038 Human
- Unigene: 284244 Human
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Alternative names
- Basic fibroblast growth factor antibody
- Basic fibroblast growth factor bFGF antibody
- BFGF antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling FGF2 with Purified ab92337 at 1:2400 dilution (0.51 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunoprecipitation - Anti-FGF2 antibody [EP1735] (ab92337)](https://www.abcam.com/ps/products/92/ab92337/Images/ab92337-10-anti-fgf2-antibody-ep1735-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-FGF2 antibody [EP1735] (ab92337)ab92337 (Purified) at 1:70 dilution (2 µg) immunoprecipitating FGF2 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+): ab92337 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92337 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Western blot - Anti-FGF2 antibody [EP1735] (ab92337)](https://www.abcam.com/ps/products/92/ab92337/Images/ab92337-367042-anti-fgf2-antibody-ep1735-westernblot-hela-human.jpg)
Western blot - Anti-FGF2 antibody [EP1735] (ab92337)Anti-FGF2 antibody [EP1735] (ab92337) at 1/5000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 17-30 kDa why is the actual band size different from the predicted?ab92337 recoginzes 3 isoforms of FGF2
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![Western blot - Anti-FGF2 antibody [EP1735] (ab92337)](https://www.abcam.com/ps/products/92/ab92337/Images/ab92337-367041-anti-fgf2-antibody-ep1735-westernblot-k562-human.jpg)
Western blot - Anti-FGF2 antibody [EP1735] (ab92337)Anti-FGF2 antibody [EP1735] (ab92337) at 1/1000 dilution (Purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 17-30 kDa why is the actual band size different from the predicted?ab92337 recoginzes 3 isoforms of FGF2
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)](https://www.abcam.com/ps/products/92/ab92337/Images/ab92337-330930-anti-fgf2-antibody-ep1735-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue sections labeling FGF2 with purified ab92337 at 1/1000 dilution (1.3 µg/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)](https://www.abcam.com/ps/products/92/ab92337/Images/ab92337-330929-anti-fgf2-antibody-ep1735-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling FGF2 with purified ab92337 at 1/1000 dilution (1.3 µg/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Sandwich ELISA - Anti-FGF2 antibody [EP1735] (ab92337)](https://www.abcam.com/ps/products/92/ab92337/Images/ab92337-107313-anti-fgf2-antibody-ep1735-sandwich-elisa.jpg)
Sandwich ELISA - Anti-FGF2 antibody [EP1735] (ab92337)Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (ab9596)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1µg/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (ab92337) at 0.5µg/ml.
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![Anti-FGF2 antibody [EP1735] (ab92337)](https://www.abcam.com/ps/products/92/ab92337/Images/ab92337-12-benefits-of-recombinant-antibodies.png)
Anti-FGF2 antibody [EP1735] (ab92337)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab92337 has been referenced in 5 publications.
- Wei F et al. Plasma endothelial cells-derived extracellular vesicles promote wound healing in diabetes through YAP and the PI3K/Akt/mTOR pathway. Aging (Albany NY) 12:12002-12018 (2020). PubMed: 32570219
- Jia ZY et al. The correlation between ultrasonographic features, bFGF, and the local invasiveness of thyroid papillary carcinoma. Medicine (Baltimore) 99:e20644 (2020). PubMed: 32590739
- Zhu W et al. Knockout of EGFL6 by CRISPR/Cas9 Mediated Inhibition of Tumor Angiogenesis in Ovarian Cancer. Front Oncol 10:1451 (2020). PubMed: 32983976
- Zhu P et al. Host Cellular Protein TRAPPC6A? Interacts with Influenza A Virus M2 Protein and Regulates Viral Propagation by Modulating M2 Trafficking. J Virol 91:N/A (2017). PubMed: 27795429
- Xiao B et al. TTF1, in the Form of Nanoparticles, Inhibits Angiogenesis, Cell Migration and Cell Invasion In Vitro and In Vivo in Human Hepatoma through STAT3 Regulation. Molecules 21:N/A (2016). PubMed: 27834930