Recombinant
RabMAb

Recombinant Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)

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Overview

  • Product name

    Anti-FGF2 antibody [EP1735] - BSA and Azide free
    See all FGF2 primary antibodies

  • Description

    Rabbit monoclonal [EP1735] to FGF2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Sandwich ELISA, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human FGF2 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: K562 cell lysate. IHC-P: Human prostate and cerebrum tissue.

  • General notes

    ab246354 is the carrier-free version of ab92337 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab246354 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab246354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 30 kDa.
IHC-P Use at an assay dependent concentration.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Sandwich ELISA Use at an assay dependent concentration.

For sandwich ELISA use this antibody as Detection at 0.5 ug/ml with Mouse monoclonal [AS24] to FGF basic (ab17279) as Capture.
IP Use at an assay dependent concentration.

Target

  • Function

    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).

  • Tissue specificity

    Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.

  • Sequence similarities

    Belongs to the heparin-binding growth factors family.

  • Post-translational
    modifications

    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

  • Cellular localization

    Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt

  • Database links

    • Alternative names

      • Basic fibroblast growth factor antibody
      • Basic fibroblast growth factor bFGF antibody
      • BFGF antibody
      • FGF 2 antibody
      • FGF B antibody
      • FGF-2 antibody
      • Fgf2 antibody
      • FGF2 basic antibody
      • FGF2_HUMAN antibody
      • FGFB antibody
      • Fibroblast growth factor 2 (basic) antibody
      • Fibroblast growth factor 2 antibody
      • Fibroblast growth factor, basic antibody
      • HBGF 2 antibody
      • HBGF-2 antibody
      • HBGF2 antibody
      • HBGH 2 antibody
      • HBGH2 antibody
      • Heparin binding growth factor 2 precursor antibody
      • Heparin-binding growth factor 2 antibody
      • Prostatropin antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling FGF2 with Purified ab92337 at 1:2400 dilution (0.51 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92337)
    • Immunoprecipitation - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)
      Immunoprecipitation - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)

      ab92337 (Purified) at 1:70 dilution (2 µg) immunoprecipitating FGF2 in HeLa whole cell lysate. 
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
      Lane 2 (+): ab92337 & HeLa whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92337 in HeLa whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92337)
      ab92337 recoginzes 3 isoforms of FGF2

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue sections labeling FGF2 with purified ab92337 at 1/1000 dilution (1.3µg/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

      Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab92337).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling FGF2 with purified ab92337 at 1/1000 dilution (1.3µg/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

      Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab92337).

    • Sandwich ELISA - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)
      Sandwich ELISA - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)

      Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (ab9596)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1µg/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (ab92337) at 0.5µg/ml.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab92337).

    References

    ab246354 has not yet been referenced specifically in any publications.

    Publishing research using ab246354? Please let us know so that we can cite the reference in this datasheet.

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