Recombinant Anti-FGF2 antibody [EPR20145-227] (ab215373)
![Western blot - Anti-FGF2 antibody [EPR20145-227] (ab215373)](https://www.abcam.com/ps/products/215/ab215373/Images/ab215373-267929-anti-fgf2-antibody-epr20145-227-western-blot.jpg "Western blot - Anti-FGF2 antibody [EPR20145-227] (ab215373)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20145-227] to FGF2
- Suitable for: WB, IP
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-FGF2 antibody [EPR20145-227]
See all FGF2 primary antibodies -
Description
Rabbit monoclonal [EPR20145-227] to FGF2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Recombinant human FGF2 active protein (aa143-288); K562, U-87 MG and SK-OV-3 whole cell lysates; Human fetal heart, fetal brain and fetal kidney lysates. IP: K562 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20145-227 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 34, 24, 22, 18 kDa (predicted molecular weight: 30 kDa).
|
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| IP |
1/30.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 34, 24, 22, 18 kDa (predicted molecular weight: 30 kDa). |
|
IP
1/30. |
Target
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Function
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107). -
Tissue specificity
Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Post-translational
modificationsPhosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. -
Cellular localization
Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. - Information by UniProt
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Database links
- Entrez Gene: 2247 Human
- Omim: 134920 Human
- SwissProt: P09038 Human
- Unigene: 284244 Human
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Alternative names
- Basic fibroblast growth factor antibody
- Basic fibroblast growth factor bFGF antibody
- BFGF antibody
see all
Images
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Western blot - Anti-FGF2 antibody [EPR20145-227] (ab215373)All lanes : Anti-FGF2 antibody [EPR20145-227] (ab215373) at 1/1000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 3 : SK-OV-3 (Human ovarian cancer cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 18,22,24,34 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2: 3 minutes; Lane 3: 1 minute.
The MW observed is consistent with the literatures (PMID:18850066, PMID: 24827991).
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![Western blot - Anti-FGF2 antibody [EPR20145-227] (ab215373)](https://www.abcam.com/ps/products/215/ab215373/Images/ab215373-267932-anti-fgf2-antibody-epr20145-227-western-blot.jpg)
Western blot - Anti-FGF2 antibody [EPR20145-227] (ab215373)Anti-FGF2 antibody [EPR20145-227] (ab215373) at 1/10000 dilution + Recombinant human FGF2 active protein (aa143-288) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-FGF2 antibody [EPR20145-227] (ab215373)](https://www.abcam.com/ps/products/215/ab215373/Images/ab215373-267928-anti-fgf2-antibody-epr20145-227-western-blot.jpg)
Western blot - Anti-FGF2 antibody [EPR20145-227] (ab215373)All lanes : Anti-FGF2 antibody [EPR20145-227] (ab215373) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 24,34 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2: 3 minutes; Lane 3: 1 minute.
The MW observed is consistent with the literatures (PMID:18850066, PMID: 24827991).
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![Immunoprecipitation - Anti-FGF2 antibody [EPR20145-227] (ab215373)](https://www.abcam.com/ps/products/215/ab215373/Images/ab215373-267927-anti-fgf2-antibody-epr20145-227-immunoprecipitation.jpg)
Immunoprecipitation - Anti-FGF2 antibody [EPR20145-227] (ab215373)FGF2 was immunoprecipitated from 0.35 mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab215373 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab215373 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution
Lane 1: K562 whole cell lysate 10µg (Input).
Lane 2: ab215373 IP in K562 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215373 in K562 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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![Anti-FGF2 antibody [EPR20145-227] (ab215373)](https://www.abcam.com/ps/products/215/ab215373/Images/ab215373-5-benefits-of-recombinant-antibodies.png)
Anti-FGF2 antibody [EPR20145-227] (ab215373)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab215373 has not yet been referenced specifically in any publications.
