Overview

  • Product name
    Anti-FGF2 antibody [FB-8]
    See all FGF2 primary antibodies
  • Description
    Mouse monoclonal [FB-8] to FGF2
  • Host species
    Mouse
  • Tested applications
    Suitable for: RIA, ELISA, Dot blot, Neutralising, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab10419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 18 kDa.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21995307

Target

  • Function
    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • Tissue specificity
    Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • Sequence similarities
    Belongs to the heparin-binding growth factors family.
  • Post-translational
    modifications
    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • Cellular localization
    Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic fibroblast growth factor antibody
    • Basic fibroblast growth factor bFGF antibody
    • BFGF antibody
    • FGF 2 antibody
    • FGF B antibody
    • FGF-2 antibody
    • Fgf2 antibody
    • FGF2 basic antibody
    • FGF2_HUMAN antibody
    • FGFB antibody
    • Fibroblast growth factor 2 (basic) antibody
    • Fibroblast growth factor 2 antibody
    • Fibroblast growth factor, basic antibody
    • HBGF 2 antibody
    • HBGF-2 antibody
    • HBGF2 antibody
    • HBGH 2 antibody
    • HBGH2 antibody
    • Heparin binding growth factor 2 precursor antibody
    • Heparin-binding growth factor 2 antibody
    • Prostatropin antibody
    see all

References

This product has been referenced in:
  • Peng WX & Wang L Adenovirus-Mediated Expression of BMP-2 and BFGF in Bone Marrow Mesenchymal Stem Cells Combined with Demineralized Bone Matrix For Repair of Femoral Head Osteonecrosis in Beagle Dogs. Cell Physiol Biochem 43:1648-1662 (2017). Read more (PubMed: 29045937) »
  • De Benedictis L  et al. Rosiglitazone reverses increased duodenal inhibitory response in spontaneously hypertensive rats. Neurogastroenterol Motil 24:e56-66 (2012). IHC-FoFr ; Rat . Read more (PubMed: 21995307) »
See all 3 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

I have received further information from lab. Unfortunately lab has confirmed that because they do not have any tested data of these antibodies being used as pairs in sELISA and also due to non availability of epitope sequence they are unable to confirm the sue of these products in sELISA.

I am sorry I am not very helpful this time. Should you have any other question please do not hesitate to contact me.

Read More

Answer

Thank you for your email.

We unfortunately have not tested any of these antibodies in sandwich ELISA so we will not be able to confirm their use. Technically, sELISA application needs two antibodies (sELISA pair) which should be raised against different part of immunogen to avoid any interference between the two; the pair should be tested in sELISA.

For these antibodies, also the immunogenic epitope sequence is not available yet so we can't recommend which ab should be used as detection or capture antibody. It seems, these antibodies are tested in indirect ELISA so we are happy to guarantee the iELISA application.

To know more about the sELISA specificity I have sent an email to my colleagues for double checking if these can be used as sELISA pairs. I will send you the information soon.

I can suggest trying these antibodies in sELISA however please note because all of these antibodies are monoclonal so either you will need to conjugate one of these antibodies or you will need an anti-mouse conjugated tertiary antibody. The positive thing, these antibodies have different isotypes so the anti mouse tertiary antibody you would need could be against a particular isotype e.g. IgG2a which should not cross react with IgG1.

Alternatively we have conjugation kits available in case you are interested in conjugating one of these antibody with HRP or other conjugates. The catalogue numbers are:

ab102892, ab102890, ab102891, ab102889 etc.

https://www.abcam.com/index.html?pageconfig=resource&rid=13148

I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

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Answer

Gracias por contactarnos de nuevo.

¿Podrías por favor indicarme el número de pedido en el que se enviaron los anticuerpos? ¿En qué estado se recibieron? ¿Nada más recibirlos se alicuotaron y guardaron a -20C?

¿En qué aplicación y especies se están usando?

Te agradezco que nos facilites estos detalles para poder entender mejor qué ha podido ocurrir para que ninguno de los tres anticuerpos funcione.

Gracias por la colaboración. Quedo a la espera de tu respuesta.

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Answer

Thank you for contacting us with your question.

We have 3 different lots in stock and as the concentration varies between these lots, I have created individual documents for each lot. If you require a document for a different lot, please let me know and I can create one for you.

We are only able to provide a certificate of compliance (CoC) for our antibodies, so I have generated these and they are attached to this email.

I hope this will be useful, but if there is anything else that we can do for you, please let me know and I'll be happy to help.

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Answer

Thank you for contacting us. We only provide certificate of compliance for our products. Please find attached the requested certificates. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your enquiry. Ab10419 is only available unconjugated. If you have any more questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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