Product nameAnti-FGF2 antibody [FB-8]
See all FGF2 primary antibodies
DescriptionMouse monoclonal [FB-8] to FGF2
Tested applicationsSuitable for: RIA, ELISA, Dot blot, Neutralising, WB, IHC-FoFrmore details
Species reactivityReacts with: Cow, Human
Recombinant full length protein (Human).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituent: 0.0268% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab10419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|RIA||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|Neutralising||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 18 kDa.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21995307|
FunctionPlays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
Tissue specificityExpressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
modificationsPhosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
Cellular localizationSecreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
- Information by UniProt
- Basic fibroblast growth factor antibody
- Basic fibroblast growth factor bFGF antibody
- BFGF antibody
This product has been referenced in:
- De Benedictis L et al. Rosiglitazone reverses increased duodenal inhibitory response in spontaneously hypertensive rats. Neurogastroenterol Motil 24:e56-66 (2012). IHC-FoFr ; Rat . Read more (PubMed: 21995307) »
- Wang L et al. A novel monoclonal antibody to fibroblast growth factor 2 effectively inhibits growth of hepatocellular carcinoma xenografts. Mol Cancer Ther 11:864-72 (2012). ELISA ; Human . Read more (PubMed: 22351746) »