Key features and details
- Rabbit polyclonal to FGF21
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FGF21 antibody
See all FGF21 primary antibodies
DescriptionRabbit polyclonal to FGF21
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to a region within amino acids 99-193 of the Human FGF21 protein (Q9NSA1).
- WB: 293T, A431, HeLa, HepG2, Molt-4, and Raji whole cell lysates. ICC/IF: HeLa cells. IHC-P: HS578T xenograft.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 22 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionStimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
- Information by UniProt
- FGF 21 antibody
- FGF-21 antibody
- Fgf21 antibody
All lanes : Anti-FGF21 antibody (ab137715) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa
12% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa labelling FGF21 with ab137715 at 1/200 (panel 1) and co-stained with a DNA probe (panel 2).
Immunohistochemical analysis of paraffin-embedded HS578T xenograft labelling FGF21 with ab137715 at 1/500.
ab137715 has not yet been referenced specifically in any publications.