Overview

  • Product name

  • Description

    Rabbit polyclonal to FGF21
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 99-193 of the Human FGF21 protein (Q9NSA1).

  • Positive control

    • WB: 293T, A431, HeLa, HepG2, Molt-4, and Raji whole cell lysates. ICC/IF: HeLa cells. IHC-P: HS578T xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab137715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 22 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.
  • Sequence similarities

    Belongs to the heparin-binding growth factors family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • FGF 21 antibody
    • FGF-21 antibody
    • Fgf21 antibody
    • FGF21_HUMAN antibody
    • FGFL antibody
    • Fibroblast growth factor 21 antibody
    • PRO10196 antibody
    • UNQ3115 antibody
    see all

Images

  • All lanes : Anti-FGF21 antibody (ab137715) at 1/1000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 22 kDa



    12% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa labelling FGF21 with ab137715 at 1/200 (panel 1) and co-stained with a DNA probe (panel 2).
  • Immunohistochemical analysis of paraffin-embedded HS578T xenograft labelling FGF21 with ab137715 at 1/500.

References

ab137715 has not yet been referenced specifically in any publications.

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