Anti-FGF21 antibody (ab64857)
Key features and details
- Rabbit polyclonal to FGF21
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FGF21 antibody
See all FGF21 primary antibodies -
Description
Rabbit polyclonal to FGF21 -
Host species
Rabbit -
Specificity
Ab64857 is not guaranteed for WB with endogenous protein since Ab64857 has been batch tested in WB using recombinant full length FGF21 protein only and not all batches detect endogenous FGF21 protein in WB. For endogenous FGR21, we recommend RabMAb ab171941 (Anti-FGF21 antibody [EPR8314(2)]) -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human FGF21.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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All applications |
Mouse
Rat
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Application | Abreviews | Notes |
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WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa). |
Target
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Function
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 26291 Human
- Entrez Gene: 56636 Mouse
- Entrez Gene: 170580 Rat
- Omim: 609436 Human
- SwissProt: Q9NSA1 Human
- SwissProt: Q9JJN1 Mouse
- Unigene: 283015 Human
- Unigene: 143736 Mouse
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Alternative names
- FGF 21 antibody
- FGF-21 antibody
- Fgf21 antibody
see all
Images
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Anti-FGF21 antibody (ab64857) at 1 µg/ml +
Recombinant human FGF21 protein (Active) (ab54141) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
ab64857 recognizes the full length FGF-21 Human Recombinant Protein which has an expected molecular weight of 19.5 kDa.
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab64857 has been referenced in 13 publications.
- Zhou L et al. MicroRNA-100-5p inhibits osteoclastogenesis and bone resorption by regulating fibroblast growth factor 21. Int J Mol Med 43:727-738 (2019). PubMed: 30535435
- Seo YJ et al. Ishige okamurae Extract Ameliorates the Hyperglycemia and Body Weight Gain of db/db Mice through Regulation of the PI3K/Akt Pathway and Thermogenic Factors by FGF21. Mar Drugs 17:N/A (2019). PubMed: 31323977
- Pan Y et al. Pancreatic fibroblast growth factor 21 protects against type 2 diabetes in mice by promoting insulin expression and secretion in a PI3K/Akt signaling-dependent manner. J Cell Mol Med N/A:N/A (2018). PubMed: 30461198
- Zhang Y et al. TFEB-dependent induction of thermogenesis by the hepatocyte SLC2A inhibitor trehalose. Autophagy 14:1959-1975 (2018). PubMed: 29996716
- Zhang J et al. Human fibroblast growth factor-21 serves as a predictor and prognostic factor in patients with hepatitis B cirrhosis combined with adrenal insufficiency. Exp Ther Med 15:3189-3196 (2018). PubMed: 29545834