Anti-FGF21 antibody (ab64857)

Reviews (4)Q&A (4)References (12)

Overview

  • Product name

    Anti-FGF21 antibody
    See all FGF21 primary antibodies

  • Description

    Rabbit polyclonal to FGF21

  • Host species

    Rabbit

  • Specificity

    Ab64857 is not guaranteed for WB with endogenous protein since Ab64857 has been batch tested in WB using recombinant full length FGF21 protein only and not all batches detect endogenous FGF21 protein in WB. For endogenous FGR21, we recommend RabMAb ab171941 (Anti-FGF21 antibody [EPR8314(2)])

  • Tested applications

    Suitable for: ELISA, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human FGF21.

    Read Abcam's proprietary immunogen policy (Peptide available as ab66562.)

  • Positive control

    • This antibody gave a positive signal on Full length FGF-21 Recombinant Protein

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. Recommended dilution / concentration to be published soon
IHC-P Use at an assay dependent concentration. PubMed: 21372207
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).

Target

Images

  • Western blot - Anti-FGF21 antibody (ab64857)
    Western blot - Anti-FGF21 antibody (ab64857)
    Anti-FGF21 antibody (ab64857) at 1 µg/ml + Recombinant human FGF21 protein (Active) (ab54141) at 0.1 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.



    ab64857 recognizes the full length FGF-21 Human Recombinant Protein which has an expected molecular weight of 19.5 kDa.

References

This product has been referenced in:

  • Zhou L  et al. MicroRNA-100-5p inhibits osteoclastogenesis and bone resorption by regulating fibroblast growth factor 21. Int J Mol Med 43:727-738 (2019). Read more (PubMed: 30535435) »
  • Seo YJ  et al. Ishige okamurae Extract Ameliorates the Hyperglycemia and Body Weight Gain of db/db Mice through Regulation of the PI3K/Akt Pathway and Thermogenic Factors by FGF21. Mar Drugs 17:N/A (2019). Read more (PubMed: 31323977) »
See all 12 Publications for this product

Publishing research using ab64857? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-8 of 8 Abreviews or Q&A

Western blot abreview for Anti-FGF21 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Recombinant protein (CHO-S cell culture product)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE gel)
Loading amount
0.5 µg
Specification
CHO-S cell culture product
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Dec 24 2019

Immunohistochemistry (Frozen sections) abreview for Anti-FGF21 antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (epithele)
Permeabilization
No
Specification
epithele
Blocking step
Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Sep 18 2019

Western blot abreview for Anti-FGF21 antibody

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% denaturing gel)
Sample
Rat Cell lysate - whole cell (hepatocytes H4IIE cell)
Specification
hepatocytes H4IIE cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Cuiqing Zhao

Verified customer

Submitted Jul 22 2013

Question

I tested the new lot GR85839 and the results are identical compared to the previous one (GR71375-1). So this is good news for me.

In additional, one of my colleague tested another lot of the same antibody (GR85321-1). This lot works even better. Could you tell me how many vials of the lot GR85321-1 you still have in stock please?

Read More
Answer

Thank you for contacting us.

I am happy that this new lot GR85839 gave good results!

With regards to lot GR85321-1, we have 56 units of this lot in stock. If you purchase over 5 units of this antibody we could give you a discount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Question

Yes it would be very nice if you can send me this vial asap. I will test it and will let you know.

Read More
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxxxxx.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Question

Good afternoon,

Here is the questionnaire filled out.

Yes I would prefer to receive the lot number GR71375-1 in a big quantity (maybe 20 vials, 20*50uL, for approximately 83 plates). A discount would be very appreciated.

I’m waiting your answer.

Read More
Answer

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

With regards to the lot GR71375-1 which gave good results, we only have 3 units left of it so we will not be able to supply you with enough of this antibody to do 83 plates. However, we have 30 units of another lot in stock which isn't GR15961-1. What I suggest is that I send you a free of charge replacement of this different lot so that you can test it in ELISA. If this antibody works, you can then purchase more vials of it for the rest of your experiments.

Please note that our antibodies are supplied in 100ul vials and that for any order of 5 or more vials, we can offer you a discount.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Read More

Question

Good afternoon,

I tested the following antibody anti-FGF21 with the lot GR15961 I received and compared with the previous lot I had (GR71375).

I obtained a big difference of signal between both.

Could you tell me if it’s still possible to order the LOT NUMBER GR71375?

Read More
Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. I have attached 3 different questionnaires so please only fill out the one which is relevant to your experiment. This information will allow us to investigate this case internally and initiate additional testing where necessary.

If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to refund or replace the antibody with an antibody from the lot GR71375 which we still have in stock in the USA.

I look forward to receiving your reply.

Read More

Western blot abreview for Anti-FGF21 antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (LIVER LYSATE)
Loading amount
100 µg
Specification
LIVER LYSATE
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12% GRADIENT GEL)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More

Abcam user community

Verified customer

Submitted Jun 17 2010

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