Anti-FGF21 antibody (ab64857)

Reviews (4)Q&A (4)References (13)
Western blot - Anti-FGF21 antibody (ab64857)

    Key features and details

    • Rabbit polyclonal to FGF21
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-FGF21 antibody
      See all FGF21 primary antibodies

    • Description

      Rabbit polyclonal to FGF21

    • Host species

      Rabbit

    • Specificity

      Ab64857 is not guaranteed for WB with endogenous protein since Ab64857 has been batch tested in WB using recombinant full length FGF21 protein only and not all batches detect endogenous FGF21 protein in WB. For endogenous FGR21, we recommend RabMAb ab171941 (Anti-FGF21 antibody [EPR8314(2)])

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human FGF21.

      Read Abcam's proprietary immunogen policy (Peptide available as ab66562.)

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab64857 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Rat
    Application Abreviews Notes
    WB (3)
    Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).

    Target

    Images

    • Western blot - Anti-FGF21 antibody (ab64857)
      Western blot - Anti-FGF21 antibody (ab64857)
      Anti-FGF21 antibody (ab64857) at 1 µg/ml + Recombinant human FGF21 protein (Active) (ab54141) at 0.1 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 22 kDa
      Observed band size: 20 kDa why is the actual band size different from the predicted?
      Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.



      ab64857 recognizes the full length FGF-21 Human Recombinant Protein which has an expected molecular weight of 19.5 kDa.

    References (13)

    Publishing research using ab64857? Please let us know so that we can cite the reference in this datasheet.

    ab64857 has been referenced in 13 publications.

    • Zhou L  et al. MicroRNA-100-5p inhibits osteoclastogenesis and bone resorption by regulating fibroblast growth factor 21. Int J Mol Med 43:727-738 (2019). PubMed: 30535435
    • Seo YJ  et al. Ishige okamurae Extract Ameliorates the Hyperglycemia and Body Weight Gain of db/db Mice through Regulation of the PI3K/Akt Pathway and Thermogenic Factors by FGF21. Mar Drugs 17:N/A (2019). PubMed: 31323977
    • Pan Y  et al. Pancreatic fibroblast growth factor 21 protects against type 2 diabetes in mice by promoting insulin expression and secretion in a PI3K/Akt signaling-dependent manner. J Cell Mol Med N/A:N/A (2018). PubMed: 30461198
    • Zhang Y  et al. TFEB-dependent induction of thermogenesis by the hepatocyte SLC2A inhibitor trehalose. Autophagy 14:1959-1975 (2018). PubMed: 29996716
    • Zhang J  et al. Human fibroblast growth factor-21 serves as a predictor and prognostic factor in patients with hepatitis B cirrhosis combined with adrenal insufficiency. Exp Ther Med 15:3189-3196 (2018). PubMed: 29545834
    View all Publications for this product

    Customer reviews and Q&As

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    1-8 of 8 Abreviews or Q&A

    Western blot abreview for Anti-FGF21 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Recombinant protein (CHO-S cell culture product)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE gel)
    Loading amount
    0.5 µg
    Specification
    CHO-S cell culture product
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 24 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-FGF21 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (epithele)
    Permeabilization
    No
    Specification
    epithele
    Blocking step
    Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 18 2019

    Western blot abreview for Anti-FGF21 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (10% denaturing gel)
    Sample
    Rat Cell lysate - whole cell (hepatocytes H4IIE cell)
    Specification
    hepatocytes H4IIE cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Cuiqing Zhao

    Verified customer

    Submitted Jul 22 2013

    Question

    I tested the new lot GR85839 and the results are identical compared to the previous one (GR71375-1). So this is good news for me.

    In additional, one of my colleague tested another lot of the same antibody (GR85321-1). This lot works even better. Could you tell me how many vials of the lot GR85321-1 you still have in stock please?

    Read More
    Answer

    Thank you for contacting us.

    I am happy that this new lot GR85839 gave good results!

    With regards to lot GR85321-1, we have 56 units of this lot in stock. If you purchase over 5 units of this antibody we could give you a discount.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Question

    Yes it would be very nice if you can send me this vial asap. I will test it and will let you know.

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxxxxx.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    Good afternoon,

    Here is the questionnaire filled out.

    Yes I would prefer to receive the lot number GR71375-1 in a big quantity (maybe 20 vials, 20*50uL, for approximately 83 plates). A discount would be very appreciated.

    I’m waiting your answer.

    Read More
    Answer

    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

    Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

    I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

    With regards to the lot GR71375-1 which gave good results, we only have 3 units left of it so we will not be able to supply you with enough of this antibody to do 83 plates. However, we have 30 units of another lot in stock which isn't GR15961-1. What I suggest is that I send you a free of charge replacement of this different lot so that you can test it in ELISA. If this antibody works, you can then purchase more vials of it for the rest of your experiments.

    Please note that our antibodies are supplied in 100ul vials and that for any order of 5 or more vials, we can offer you a discount.

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Question

    Good afternoon,

    I tested the following antibody anti-FGF21 with the lot GR15961 I received and compared with the previous lot I had (GR71375).

    I obtained a big difference of signal between both.

    Could you tell me if it’s still possible to order the LOT NUMBER GR71375?

    Read More
    Answer

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. I have attached 3 different questionnaires so please only fill out the one which is relevant to your experiment. This information will allow us to investigate this case internally and initiate additional testing where necessary.

    If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to refund or replace the antibody with an antibody from the lot GR71375 which we still have in stock in the USA.

    I look forward to receiving your reply.

    Read More

    Western blot abreview for Anti-FGF21 antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (LIVER LYSATE)
    Loading amount
    100 µg
    Specification
    LIVER LYSATE
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-12% GRADIENT GEL)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 17 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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