Overview

  • Product name
    Anti-FGF21 antibody
    See all FGF21 primary antibodies
  • Description
    Goat polyclonal to FGF21
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide:

    CQRPDGALYGSLH

    , corresponding to internal sequence amino acids 103-115 of Human FGF21 (NP_061986.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human Liver lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 0.5% Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab77692 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/2000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.
    • Sequence similarities
      Belongs to the heparin-binding growth factors family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
      • Alternative names
        • FGF 21 antibody
        • FGF-21 antibody
        • Fgf21 antibody
        • FGF21_HUMAN antibody
        • FGFL antibody
        • Fibroblast growth factor 21 antibody
        • PRO10196 antibody
        • UNQ3115 antibody
        see all

      Images

      • Western blot - Anti-FGF21 antibody (ab77692)
        Western blot - Anti-FGF21 antibody (ab77692)
        Anti-FGF21 antibody (ab77692) at 1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg

        Predicted band size: 22 kDa
        Observed band size: 22 kDa



        Primary incubation was 1 hour.

      References

      ab77692 has not yet been referenced specifically in any publications.

      Publishing research using ab77692? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1 Review or Q&A

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      Votes: Highest First

      I am interested in a couple of your FGF21 antibodies. It looks like two of them have been tested in an ELISA format, ab64857 and ab77692. Are these antibodies used for capture or detection. What is the detection limit for these antibodies.

      Thank you for contacting us. These antibodies have both been tested in a straight ELISA, not in a sandwich ELISA and do not have matched pairs. The detection limit of ab77962 is 1/2,000, for ab64857 it is 1/20,000. Please let me know if you have any fu...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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