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Anti-FGF21 antibody (ab77692)

Reviews (1)Q&A (1)

Key features and details

  • Goat polyclonal to FGF21
  • Suitable for: ELISA, WB
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-FGF21 antibody [EPR8314(2)] (ab171941)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-FGF21 antibody
    See all FGF21 primary antibodies

  • Description

    Goat polyclonal to FGF21

  • Host species

    Goat

  • Tested applications

    Suitable for: ELISA, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee

  • Immunogen

    Synthetic peptide within Human FGF21 aa 100-200 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Accession: NP_061986.1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human Liver lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 0.5% Tris buffered saline
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab77692 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab77692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

Target

  • Function

    Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.

  • Sequence similarities

    Belongs to the heparin-binding growth factors family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • FGF 21 antibody
      • FGF-21 antibody
      • Fgf21 antibody
      • FGF21_HUMAN antibody
      • FGFL antibody
      • Fibroblast growth factor 21 antibody
      • PRO10196 antibody
      • UNQ3115 antibody
      see all

    References (0)

    Publishing research using ab77692? Please let us know so that we can cite the reference in this datasheet.

    ab77692 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-FGF21 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Recombinant protein (CHO-S)
    Gel Running Conditions
    Reduced Denaturing (8% gel)
    Loading amount
    0.5 µg
    Specification
    CHO-S
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Read More

    MRS. Min Sun Kim

    Verified customer

    Submitted Sep 30 2020

    Question

    I am interested in a couple of your FGF21 antibodies. It looks like two of them have been tested in an ELISA format, ab64857 and ab77692. Are these antibodies used for capture or detection. What is the detection limit for these antibodies.

    Read More
    Answer

    Thank you for contacting us. These antibodies have both been tested in a straight ELISA, not in a sandwich ELISA and do not have matched pairs. The detection limit of ab77962 is 1/2,000, for ab64857 it is 1/20,000. Please let me know if you have any further questions.

    Read More

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