Recombinant
RabMAb

Anti-FGF21 antibody [EPR8314(2)] (ab171941)

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Overview

  • Product name
    Anti-FGF21 antibody [EPR8314(2)]
    See all FGF21 primary antibodies
  • Description
    Rabbit monoclonal [EPR8314(2)] to FGF21
  • Host species
    Rabbit
  • Specificity
    The immunogen used for this product shares 6 continuous identical amino acids with SIKE1. Cross-reactivity with this protein has not been confirmed experimentally
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FGF21 aa 1-100. The exact sequence is proprietary.
    Database link: Q9NSA1

  • Positive control
    • Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556) can be used as a positive control in WB. Human fetal liver lysate, HeLa cell lysate, A549 cell lysate, Human lipoma tissue, Human thyroid carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Allow to warm to room temp and agitate gently before aliquotting. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR8314(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 22 kDa.
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    Images

    • Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/1000 dilution (purified) + Mouse spleen tissue lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 22 kDa
      Observed band size: 22 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution (purified) + Rat spleen tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 22 kDa
      Observed band size: 22 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution (purified) + Human fetal liver tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 22 kDa
      Observed band size: 22 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/1000 dilution (purified) + HeLa cell lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 22 kDa
      Observed band size: 22 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Immunohistochemical staining of paraffin embedded human stomach with purified ab171941 at a working dilution of 1/250. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      All lanes : Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal liver tissue lysate
      Lane 2 : Hela cell line lysate
      Lane 3 : A549 cell line lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 22 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] (ab171941)

      Immunohistochemical analysis of paraffin embedded Human lipoma tissue labeling FGF21 with unpurified ab171941 at 1/100 .

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] (ab171941)

      Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labeling FGF21 with unpurified ab171941 at 1/100 .

    References

    This product has been referenced in:
    • Vargas D  et al. Thermogenic capacity of human periaortic adipose tissue is transformed by body weight. PLoS One 13:e0194269 (2018). Read more (PubMed: 29554135) »
    • Yan K  et al. Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21. Front Physiol 9:191 (2018). Read more (PubMed: 29593555) »
    See all 22 Publications for this product

    Publishing research using ab171941? Please let us know so that we can cite the reference in this datasheet.

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