Recombinant
RabMAb

Recombinant Anti-FGF21 antibody [EPR8314(2)] (ab171941)

Reviews (1) Submit a question References (38)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8314(2)] to FGF21
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FGF21 antibody [EPR8314(2)]
    See all FGF21 primary antibodies

  • Description

    Rabbit monoclonal [EPR8314(2)] to FGF21

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 6 continuous identical amino acids with SIKE1. Cross-reactivity with this protein has not been confirmed experimentally.

    Expression levels of the target protein vary with sample type and some optimisation may be required (PMID: 27285327).  For western blot using cell lines, it may be necessary to collect cell culture supernatant for endogenous FGF21 detection as the target protein is readily secreted (PMID: 24041694; PMID: 26691139).

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human FGF21 aa 1-100. The exact sequence is proprietary.
    Database link: Q9NSA1

  • Positive control

    • Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556) can be used as a positive control in WB. Human fetal liver lysate, A549 cell lysate, Human lipoma tissue, Human thyroid carcinoma tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Allow to warm to room temp and agitate gently before aliquotting. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8314(2)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 22 kDa.
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    Images

    • Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/1000 dilution (purified) + Mouse spleen tissue lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 22 kDa
      Observed band size: 22 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution (purified) + Rat spleen tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 22 kDa
      Observed band size: 22 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution (purified) + Human fetal liver tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 22 kDa
      Observed band size: 22 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Immunohistochemical staining of paraffin embedded human stomach with purified ab171941 at a working dilution of 1/250. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      All lanes : Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal liver tissue lysate
      Lane 2 : Hela cell line lysate
      Lane 3 : A549 cell line lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 22 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] (ab171941)

      Immunohistochemical analysis of paraffin embedded Human lipoma tissue labeling FGF21 with unpurified ab171941 at 1/100 .

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] (ab171941)

      Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labeling FGF21 with unpurified ab171941 at 1/100 .

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    Publishing research using ab171941? Please let us know so that we can cite the reference in this datasheet.

    ab171941 has been referenced in 38 publications.

    • Hidalgo-Gutiérrez A  et al. ß-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9R239X mice. EMBO Mol Med 11:N/A (2019). PubMed: 30482867
    • Fujii N  et al. Impact of aging and caloric restriction on fibroblast growth factor 21 signaling in rat white adipose tissue. Exp Gerontol 118:55-64 (2019). PubMed: 30620889
    • Liu J  et al. Liver-derived fibroblast growth factor 21 mediates effects of glucagon-like peptide-1 in attenuating hepatic glucose output. EBioMedicine 41:73-84 (2019). PubMed: 30827929
    • Zhang Q  et al. Lack of muscle mTOR kinase activity causes early onset myopathy and compromises whole-body homeostasis. J Cachexia Sarcopenia Muscle 10:35-53 (2019). PubMed: 30461220
    • Sun M  et al. Fibroblast growth factor 21 protects against pathological cardiac remodeling by modulating galectin-3 expression. J Cell Biochem 120:19529-19540 (2019). PubMed: 31286550
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-FGF21 antibody [EPR8314(2)]

     Average
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (heart)
    Gel Running Conditions
    Non-reduced Denaturing (15%)
    Loading amount
    30 µg
    Specification
    heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 25 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com