Key features and details
- Rabbit polyclonal to FGF22
- Suitable for: ELISA, WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FGF22 antibody
DescriptionRabbit polyclonal to FGF22
Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from internal sequence of human FGF22.
- Human ovary tissue. Extracts from Jurkat cells and HT-29 cells. MCF-7 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 19 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/500 - 1/1000.|
FunctionMay be involved in hair development.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
- Information by UniProt
- FGF 22 antibody
- FGF-22 antibody
- FGF22 antibody
All lanes : Anti-FGF22 antibody (ab74860) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from HT-29 cells
Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 19 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry analysis of FGF22 in paraffin-embedded human ovary tissue using ab74860, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Immunofluorescence analysis of FGF22 in MCF-7 cells using ab74860, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
ab74860 has been referenced in 1 publication.
- Castilho ACS et al. Expression of fibroblast growth factor 22 (FGF22) and its receptor, FGFR1B, during development and regression of bovine corpus luteum. Theriogenology 125:1-5 (2019). PubMed: 30366151