Overview

  • Product name

    Anti-FGF22 antibody
  • Description

    Rabbit polyclonal to FGF22
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from internal sequence of human FGF22.

  • Positive control

    • Human ovary tissue. Extracts from Jurkat cells and HT-29 cells. MCF-7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/60000.
WB 1/500 - 1/1000. Predicted molecular weight: 19 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

Images

  • All lanes : Anti-FGF22 antibody (ab74860) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from HT-29 cells
    Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 19 kDa
    Observed band size: 21 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 55 kDa, 85 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry analysis of FGF22 in paraffin-embedded human ovary tissue using ab74860, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • Immunofluorescence analysis of FGF22 in MCF-7 cells using ab74860, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

References

This product has been referenced in:

  • Castilho ACS  et al. Expression of fibroblast growth factor 22 (FGF22) and its receptor, FGFR1B, during development and regression of bovine corpus luteum. Theriogenology 125:1-5 (2019). Read more (PubMed: 30366151) »
See 1 Publication for this product

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