Overview

  • Product name

    Anti-FGF3 antibody [MSD1]
    See all FGF3 primary antibodies
  • Description

    Mouse monoclonal [MSD1] to FGF3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide: RRTQKSSLFLPRVL. FGF3 conjugated to BSA, corresponding to amino acids 170-183 of Human FGF3.

Properties

Applications

Our Abpromise guarantee covers the use of ab10830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Use at an assay dependent dilution. Fixation in 4% paraformaldehyde in PBS for 20 min.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 29 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Fibroblast growth factors (FGFs) can stimulate the proliferation of mesenchymal, epithelial and neuroectodermal cells. FGF3 belongs to the heparin binding growth factors family. Members of the family include Acidic FGF (also known as aFGF or FGF 1), basic FGF (bFGF or FGF 2), FGF 3 (also known as Int2) and FGF 4 (also known as hst or Kaposi FGF). FGF 4 was first revealed to be an oncoprotein in Kaposi's sarcoma cells. Other family members include FGF 5, FGF 6, FGF 7 (keratinocyte growth factor, KGF), FGF 8 (AIGF), FGF 9 (GAF) and FGF 10. FGF3 is activated by proviral insertion of mouse mammary tumor virus (MMTV). FGF3 is responsible for most of breast malignancies.
    • Cellular localization

      Secreted.
    • Database links

    • Alternative names

      • FGF 3 antibody
      • Fibroblast growth factor 3 antibody
      • HBGF 3 antibody
      • HBGF3 antibody
      • INT 2 antibody
      • INT 2 proto oncogene protein antibody
      • INT 2 proto oncogene protein precurosr antibody
      • INT2 antibody
      • INT2 proto oncogene protein precurosr antibody
      • Murine mammary tumor virus integration site (v int 2) oncogene homolog antibody
      • Murine mammary tumor virus integration site 2 antibody
      • Oncogene INT2 antibody
      • V INT2 murine mammary tumor virus integration site oncogene homolog antibody
      see all

    References

    This product has been referenced in:

    • Gao Q  et al. The signalling receptor MCAM coordinates apical-basal polarity and planar cell polarity during morphogenesis. Nat Commun 8:15279 (2017). Read more (PubMed: 28589943) »
    • Xiao W  et al. Upregulation of miR-31* Is Negatively Associated with Recurrent/Newly Formed Oral Leukoplakia. PLoS One 7:e38648 (2012). WB ; Human . Read more (PubMed: 22719913) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (retina)
    Permeabilization
    Yes - Triton X 100
    Specification
    retina
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Ms. Danye Jiang

    Verified customer

    Submitted May 29 2019

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Unerupted Tooth Bud)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citric Acid Monobasic pH6
    Permeabilization
    No
    Specification
    Unerupted Tooth Bud
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Mar 14 2017

    Answer

    Thank you for getting back to me. I appreciate your frustration. Please let me apologise for the lack of a recommended dilution for this antibody by immunofluorescence and the cited publication which I conceded does not reference this antibody. I have updated the datasheet with the correct reference: Antoine M, Daum M, Kohl R, Blecken V, Close MJ, Peters G, Kiefer P. NH2-terminal cleavage of xenopus fibroblast growth factor 3 is necessary for optimal biological activity and receptor binding. Cell Growth Differ. 2000 Nov;11(11):593-605. PMID: 11095248 In the reference they refer to the antibody as "MSDI" as this precedes the sourcing of this antibody by Abcam. I have emailed this paper to you as a follow up e-mail. According to the reference that I have been supplied with this antibody was tested using paraformaldehyde fixed cos-1 cells. Therefore the antibody is yet to be tested using IHC. That is not to say that it will not work, just that it has not been tested. I am pleased that you are able to optimise this antibody by setting up a range of concentrations. However, should you have difficulties with this antibody please do not hesitate to get back in touch with us. I appreciate your patience in this matter and wish you best of luck in your research.

    Read More

    Answer

    Thank you for your enquiry. I am sorry but we do not have any further information as to a recommended dilution for this antibody. I would like to recommend that you contact Michael I Koukourakis given that they applied this antibody. According to the following publication he can be contacted on: targ@her.forthnet.gr Giatromanolaki A et al. DEC1 (STRA13) protein expression relates to hypoxia- inducible factor 1-alpha and carbonic anhydrase-9 overexpression in non-small cell lung cancer. J Pathol 200:222-8 (2003). PubMed: 12754744 I am sorry that I cannot be of more assistance on this occasion.

    Read More

    Question
    Answer

    Thank you for your enquiry and your patience in awaiting a response. Unfortunately, we are not aware of a tissue that expresses reasonable levels of Fgf3.The antibody has been tested on whole embryos, as they express Fgf-3 (as determined by in situ) at several sites but were never able to obtain convincing results. It is generally not expressed in adult tissues. Therefore, I think the only control would be recombinant protein. If you need any further assistance please do not hesitate to get back in touch with us.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up