Product nameAnti-FGF5 antibody
See all FGF5 primary antibodies
DescriptionRabbit polyclonal to FGF5
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rabbit, Cow, Dog, Human
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FGF5.
- Recombinant human FGF5 protein (ab50229) can be used as a positive control in WB. This anitbody gave a positive signal in the following tissues lysates: E14 Mouse Embryo Brain; E16 Ms Embryo Brain; E18 Ms Embryo Brain This antibody gave a positive result when used in the following formaldehyde fixed cell lines: NIH-3T3.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab88118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionFunctions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase.
Tissue specificityExpressed in neonatal brain.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
Developmental stageCan transform NIH 3T3 cells.
- Information by UniProt
- FGF 5 antibody
- FGF-5 antibody
- FGF5 antibody
ICC/IF image of ab88118 stained NIH-3T3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab88118 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-FGF5 antibody (ab88118) at 1 µg/ml
Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
Lane 2 : E16 Ms Embryo Brain Tissue Lysate
Lane 3 : E18 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 125 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes