Key features and details
- Rabbit polyclonal to FGF8
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-FGF8 antibody
See all FGF8 primary antibodies
DescriptionRabbit polyclonal to FGF8
SpecificityNo cross reactivity with other proteins.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
A synthetic peptide corresponding to a sequence at the C-terminal of Human FGF8.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab81384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.|
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionStimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells.
Involvement in diseaseDefects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6) [MIM:612702]. Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous.
Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
Developmental stageIn adults expression is restricted to the gonads.
- Information by UniProt
- AIGF antibody
- Androgen induced growth factor antibody
- Androgen-induced growth factor antibody
ab81384 staining FGF8 in Human Ovarian cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 37°C; antigen retrieval was by microwave heat mediation in a citrate buffer. Samples were incubated with primary antibody (2 μg/mL) for 2 hours at 37°C. A Biotin-conjugated secondary antibody was used.
ab81384 at 1µg/ml staining FGF8 in Rat ovary tissue sections by Immunohistochemistry(Formalin/ PFA-fixed paraffin-embedded sections). The tissue underwent heat mediated antigen retrieval. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.
All lanes : Anti-FGF8 antibody (ab81384)
All lanes : Rat Ovary Tissue Lysate
Predicted band size: 27 kDa
ab81384 has been referenced in 3 publications.
- Fex Svenningsen Å et al. Macrophage migration inhibitory factor (MIF) modulates trophic signaling through interaction with serine protease HTRA1. Cell Mol Life Sci 74:4561-4572 (2017). ICC ; Mouse . PubMed: 28726057
- Pei Y et al. FGF8 promotes cell proliferation and resistance to EGFR inhibitors via upregulation of EGFR in human hepatocellular carcinoma cells. Oncol Rep 38:2205-2210 (2017). PubMed: 28791365
- Hetzl AC et al. Fibroblast growth factor, estrogen, and prolactin receptor features in different grades of prostatic adenocarcinoma in elderly men. Microsc Res Tech 76:321-30 (2013). PubMed: 23362007