Recombinant
RabMAb

Recombinant Anti-FGF9/GAF antibody [EPR19937] (ab206408)

Overview

  • Product name

    Anti-FGF9/GAF antibody [EPR19937]
    See all FGF9/GAF primary antibodies
  • Description

    Rabbit monoclonal [EPR19937] to FGF9/GAF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human FGF9/GAF aa 1 to the C-terminus. The exact sequence is proprietary. Mature protein without the proprotein from aa 1-3.
    Database link: P31371

  • Positive control

    • WB: Human FGF9/GAF active protein; human fetal brain, fetal kidney and ovary cancer lysates; mouse kidney and brain lysates; rat brain lysate; C6, PC-12, NIH/3T3 and U-87 MG whole cell lysates.
  • General notes

    Previously labelled as FGF9.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab206408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).

Target

  • Function

    May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
  • Tissue specificity

    Glial cells.
  • Involvement in disease

    Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3) [MIM:612961]. Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness.
  • Sequence similarities

    Belongs to the heparin-binding growth factors family.
  • Post-translational
    modifications

    Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.
    N-glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • FGF 9 antibody
    • FGF-9 antibody
    • FGF9 antibody
    • FGF9_HUMAN antibody
    • Fibroblast growth factor 9 antibody
    • GAF (Glia-activafibroblast growth factor 9 (glia-activating factor) antibody
    • GAF antibody
    • Glia Activating Factor antibody
    • Glia-activating factor antibody
    • HBFG 9 antibody
    • HBFG9 antibody
    • HBGF-9 antibody
    • Heparin-binding growth factor 9 antibody
    • MGC119914 antibody
    • MGC119915 antibody
    • SYNS3 antibody
    see all

Images

  • All lanes : Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution

    Lane 1 : Human FGF9/GAF active protein
    Lane 2 : Human FGF16 recombinant protein
    Lane 3 : Human FGF20 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1/2: 1 seconds; Lane 3: 15 seconds.

    Human FGF9/GAF active protein contains aa1-208; FGF16 and FGF20 recombinant protein contain aa1-203 and aa4-208 respectively, both with His-Tag®. 

  • All lanes : Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human ovary cancer lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 23 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1/2: 15 seconds; Lane 3: 30 seconds.

    The MW observed is consistent with the literature (PMID:16452189, PMID: 25925261).

  • All lanes : Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1/2: 15 seconds; Lane 3: 30 seconds.

    The MW observed is consistent with the literature (PMID:16452189, PMID: 25925261).

  • All lanes : Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1-3: 15 seconds; Lane 4: 3 minutes.

    The MW observed is consistent with the literature (PMID:16452189, PMID: 25925261).

References

ab206408 has not yet been referenced specifically in any publications.

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