Anti-FGFR1 alpha antibody [M2F12] (ab829)
- Datasheet
- References (8)
- Protocols
Overview
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Product nameAnti-FGFR1 alpha antibody [M2F12]
See all FGFR1 alpha primary antibodies -
DescriptionMouse monoclonal [M2F12] to FGFR1 alpha
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Host speciesMouse
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SpecificityReacts with alpha isoform of FRFR1.
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Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, IP, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant human ectodomain of FGFr1a expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
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EpitopeNH2-terminus of unique NH2-terminal Ig loop of FGFr1. Epitope is within the sequence between glu30 and ala74 of FGFr1a.
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Positive control
- IHC: Rat prostate tissue.
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General notes
This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium azide
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Concentration information loading... -
PurityProtein G purified
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Purification notesPurified from TCS
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ClonalityMonoclonal
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Clone numberM2F12
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IsotypeIgG2a
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| ICC/IF | Use a concentration of 10 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 19728793 | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 120 kDa. |
Target
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RelevanceFGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family and contains an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is widely expressed, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.
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Cellular localizationCell Membrane
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Database links
- Entrez Gene: 2260 Human
- Entrez Gene: 14182 Mouse
- Entrez Gene: 79114 Rat
- Omim: 136350 Human
- SwissProt: P11362 Human
- SwissProt: P16092 Mouse
- SwissProt: Q04589 Rat
- Unigene: 264887 Human
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Alternative names
- BFGFR antibody
- CD331 antibody
- CEK antibody
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Images
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![Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 alpha antibody [M2F12] (ab829)](https://www.abcam.com/ps/products/0/ab829/Images/FGFR1-alpha-Primary-antibodies-ab829-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 alpha antibody [M2F12] (ab829)ICC/IF image of ab829 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab829, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Mao Y et al. VEGF-A/VEGFR-2 and FGF-2/FGFR-1 but not PDGF-BB/PDGFR-ß play important roles in promoting immature and inflammatory intraplaque angiogenesis. PLoS One 13:e0201395 (2018). Read more (PubMed: 30125282) »
- Nakamichi M et al. Basic Fibroblast Growth Factor Induces Angiogenic Properties of Fibrocytes to Stimulate Vascular Formation during Wound Healing. Am J Pathol 186:3203-3216 (2016). Read more (PubMed: 27773739) »
