For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    fgfr1-alpha-antibody-m2f12-ab829.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Angiogenesis Growth Factors FGF FGF Receptors
Share by email

Anti-FGFR1 alpha antibody [M2F12] (ab829)

  • Datasheet
Reviews (2)Q&A (3)References (12)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 alpha antibody [M2F12] (ab829)

    Key features and details

    • Mouse monoclonal [M2F12] to FGFR1 alpha
    • Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, IP
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG2a

    You may also be interested in

    Primary
    Product image
    Anti-FGFR2 antibody (ab10648)
    Primary
    Product image
    Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474) antibody [EPR18853] (ab192623)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-FGFR1 alpha antibody [M2F12]
      See all FGFR1 alpha primary antibodies
    • Description

      Mouse monoclonal [M2F12] to FGFR1 alpha
    • Host species

      Mouse
    • Specificity

      Reacts with alpha isoform of FRFR1.
    • Tested applications

      Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, IPmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Human
    • Immunogen

      Recombinant fragment corresponding to Human FGFR1 alpha. Recombinant human ectodomain of FGFr1a expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.

    • Epitope

      NH2-terminus of unique NH2-terminal Ig loop of FGFr1. Epitope is within the sequence between glu30 and ala74 of FGFr1a.
    • Positive control

      • IHC: Rat prostate tissue.
    • General notes

      This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      Purified from TCS
    • Clonality

      Monoclonal
    • Clone number

      M2F12
    • Isotype

      IgG2a
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Growth Factors
      • FGF
      • FGF Receptors
      • Signal Transduction
      • Growth Factors/Hormones
      • FGF
      • Cancer
      • Growth factors
      • FGF
      • Neuroscience
      • Development

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant human FGFR1 alpha protein (Fc Chimera) (ab55758)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab829 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P (1)
    Use at an assay dependent concentration. PubMed: 19728793
    WB (1)
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    Use a concentration of 10 µg/ml.
    IP
    Use at an assay dependent concentration.
    Notes
    IHC-P
    Use at an assay dependent concentration. PubMed: 19728793
    WB
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    Use a concentration of 10 µg/ml.
    IP
    Use at an assay dependent concentration.

    Target

    • Relevance

      FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family and contains an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is widely expressed, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.
    • Cellular localization

      Cell Membrane
    • Database links

      • Entrez Gene: 2260 Human
      • Entrez Gene: 14182 Mouse
      • Entrez Gene: 79114 Rat
      • Omim: 136350 Human
      • SwissProt: P11362 Human
      • SwissProt: P16092 Mouse
      • SwissProt: Q04589 Rat
      • Alternative names

        • BFGFR antibody
        • CD331 antibody
        • CEK antibody
        • FGFBR antibody
        • FGFR1 antibody
        • FLG antibody
        • FLT2 antibody
        • FMS like tyrosine kinase 2 antibody
        • HBGFR antibody
        • KAL2 antibody
        • N SAM antibody
        • OGD antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 alpha antibody [M2F12] (ab829)
        Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 alpha antibody [M2F12] (ab829)

        ICC/IF image of ab829 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab829, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

        This image was generated using the ascites version of the product. 

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (12)

      Publishing research using ab829? Please let us know so that we can cite the reference in this datasheet.

      ab829 has been referenced in 12 publications.

      • Formisano L  et al. Aberrant FGFR signaling mediates resistance to CDK4/6 inhibitors in ER+ breast cancer. Nat Commun 10:1373 (2019). PubMed: 30914635
      • Zhang GY  et al. Thyroxine (T4) may promote re-epithelialisation and angiogenesis in wounded human skin ex vivo. PLoS One 14:e0212659 (2019). PubMed: 30925152
      • Voytyuk I  et al. BACE2 distribution in major brain cell types and identification of novel substrates. Life Sci Alliance 1:e201800026 (2018). PubMed: 30456346
      • Mao Y  et al. VEGF-A/VEGFR-2 and FGF-2/FGFR-1 but not PDGF-BB/PDGFR-ß play important roles in promoting immature and inflammatory intraplaque angiogenesis. PLoS One 13:e0201395 (2018). PubMed: 30125282
      • Nakamichi M  et al. Basic Fibroblast Growth Factor Induces Angiogenic Properties of Fibrocytes to Stimulate Vascular Formation during Wound Healing. Am J Pathol 186:3203-3216 (2016). PubMed: 27773739
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review

      Filter by Application

      Filter by Species

      Filter by Ratings

      1-2 of 2 Abreviews

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FGFR1 alpha antibody [M2F12]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Formalin Fixed Paraffin Embedded)
      Permeabilization
      No
      Specification
      Formalin Fixed Paraffin Embedded
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Nov 12 2018

      Western blot abreview for Anti-FGFR1 alpha antibody [M2F12]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Neuronal cells)
      Gel Running Conditions
      Non-reduced Denaturing (4-12%)
      Loading amount
      25 µg
      Specification
      Neuronal cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Dec 19 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2022 Abcam plc. All rights reserved.