Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
![Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)](https://www.abcam.com/ps/products/134/ab134043/Images/ab134043-148139-anti-fgfr1-alpha-phospho-y654-antibody-ep8432-western-blot.jpg "Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)")
Key features and details
- Rabbit monoclonal [EP843(2)] to FGFR1 alpha (phospho Y654)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)]
See all FGFR1 alpha primary antibodies -
Description
Rabbit monoclonal [EP843(2)] to FGFR1 alpha (phospho Y654) -
Host species
Rabbit -
Specificity
Only detects FGFR1 alpha when phosphorylated at Tyrosine 654 -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
A phospho specific peptide corresponding to residues surrounding Tyrosine 654 of Human FGFR1 alpha.
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Positive control
- Constitutively active FGFR1
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General notes
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.60 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP843(2) -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 130 kDa. |
Target
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Relevance
FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family and contains an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is widely expressed, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 14182 Mouse
- SwissProt: P16092 Mouse
- Unigene: 265716 Mouse
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Alternative names
- BFGFR antibody
- CD331 antibody
- CEK antibody
see all
Images
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Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)All lanes : Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043) at 1/1000 dilution
Lane 1 : Control
Lane 2 : Constitutively active FGFR1 alpha
Lane 3 : Kinase dead lysates from Xenopus oocytes
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-
rabbit at 1/2000 dilution
Predicted band size: 130 kDa -
![OI-RD Scanning - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)](https://www.abcam.com/ps/products/134/ab134043/Images/ab134043-148138-anti-fgfr1-alpha-phospho-y654-antibody-ep8432.jpg)
OI-RD Scanning - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
Datasheets and documents
References (0)
ab134043 has not yet been referenced specifically in any publications.
