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RabMAb

Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

Submit a review Q&A (1)
Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
  • OI-RD Scanning - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
  • Dot Blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
  • Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
  • Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
  • Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
  • Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

Key features and details

  • Rabbit monoclonal [EP843(2)] to FGFR1 alpha (phospho Y654)
  • Suitable for: WB, Dot blot, Indirect ELISA
  • Isotype: IgG

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)]
    See all FGFR1 alpha primary antibodies

  • Description

    Rabbit monoclonal [EP843(2)] to FGFR1 alpha (phospho Y654)

  • Host species

    Rabbit

  • Specificity

    This antibody recognizes FGFR1 (phospho Y654), FGFR2 (phospho Y657), FGFR3 (phospho Y648) and FGFR4 (phospho Y643).

  • Tested applications

    Suitable for: WB, Dot blot, Indirect ELISAmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity


    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Constitutively active FGFR1 alpha

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 130 kDa.
Dot blot
1/1000.
Indirect ELISA
Use at an assay dependent concentration.

1000 ng/ml
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 130 kDa.
Dot blot
1/1000.
Indirect ELISA
Use at an assay dependent concentration.

1000 ng/ml
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Target

  • Relevance

    FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family and contains an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is widely expressed, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.

  • Cellular localization

    Cell Membrane

  • Database links

    • Alternative names

      • BFGFR antibody
      • CD331 antibody
      • CEK antibody
      • FGFBR antibody
      • FGFR1 antibody
      • FLG antibody
      • FLT2 antibody
      • FMS like tyrosine kinase 2 antibody
      • HBGFR antibody
      • KAL2 antibody
      • N SAM antibody
      • OGD antibody
      see all

    Images

    • Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
      Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
      All lanes : Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043) at 1/1000 dilution

      Lane 1 : Control
      Lane 2 : Constitutively active FGFR1 alpha
      Lane 3 : Kinase dead lysates from Xenopus oocytes

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-

      rabbit at 1/2000 dilution

      Predicted band size: 130 kDa

    • OI-RD Scanning - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
      OI-RD Scanning - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Dot Blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
      Dot Blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

      Dot blot analysis of ab134043 at 1/1000 dilution. The secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution.

      Lane 1: FGFR1 non-phospho peptide
      Lane 2: FGFR1 Y653 phospho peptide
      Lane 3: FGFR1 Y654 phospho peptide
      Lane 4: FGFR1 Y653 + Y654 phospho peptide
      Lane 5: FGFR2 non-phospho peptide
      Lane 6: FGFR2 Y656 phospho peptide
      Lane 7: FGFR2 Y657 phospho peptide
      Lane 8: FGFR2 Y656 + Y657 phospho peptide
      Lane 9: FGFR3 non-phospho peptide
      Lane 10: FGFR3 Y647 phospho peptide
      Lane 11: FGFR3 Y648 phospho peptide
      Lane 12: FGFR3 Y647 + Y648 phospho peptide
      Lane 13: FGFR4 non-phospho peptide
      Lane 14: FGFR4 Y642 phospho peptide
      Lane 15: FGFR4 Y643 phospho peptide
      Lane 16: FGFR4 Y642 + Y643 phospho peptide

    • Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
      Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

      Indirect ELISA analysis of ab134043 at 1/1000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

      Substrate solution: p-nitrophenyl phosphate(PNPP).

    • Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
      Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

      Indirect ELISA analysis of ab134043 at 1/1000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

      Substrate solution: p-nitrophenyl phosphate(PNPP).

    • Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
      Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

      Indirect ELISA analysis of ab134043 at 1/1000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

      Substrate solution: p-nitrophenyl phosphate(PNPP).

    • Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
      Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

      Indirect ELISA analysis of ab134043 at 1/1000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

      Substrate solution: p-nitrophenyl phosphate(PNPP).

    References (0)

    Publishing research using ab134043? Please let us know so that we can cite the reference in this datasheet.

    ab134043 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Hello, I would like to know if this antibody is specific against the phosphorylated form of FGFR1 or if it cross-reacts with the phosphorylated forms of the other FGFRs. Also, I am interested in antibodies against pFGFR1 and pFGFR4 which do no cross-react with the other FGFRs. Could you please let me know if you have any of those? Thanks.

    Read More
    Answer

    Thank you for contacting us.

    I have conducted a search through our catalogue for antibodies against pFGFR1 and pFGFR4 which do no cross-react with the other FGFRs.

    Ab59180 is specific against FGFR1 when it is phosphorilated at Tyr766. Similarly, ab59194 is specific against FGFR1 when it is phosphorilated at Tyr654, while ab52165 is specific against FGFR1 when it is phosphorilated at Tyr154.

    These three antibodies do not bind to non-phosphorilated proteins. If you would send me the accession numbers or the exact protein sequences of other FGFRs that you are concerned with cross reactivity, I can align them with our immunogen sequence for sequence homology and possible cross reactivity.

    We do have other alternatives (ab111124 and ab134043) against pFGFR1. However, these antibodies may also detect FGFR2, FGFR3 and FGFR4 phosphorilated at the equivalent sites. Although the antibodies have not been tested to determine if it detects these other endogenous forms, based on sequence homology reactivity would be expected.

    Unfortunately, all the https://www.abcam.com/Search?Keywords=FGFR4+&fReset=1&pt=1&source=TopSearch&btnSearch=Search are against non phosphorilated forms, so I am afraid they would not fit your requirements.

    Please do not hesitate to let me know whether you require a proforma, or any further assistance.

    Read More

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