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RabMAb

Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

  • Datasheet
Submit a review Q&A (1)

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Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
  • OI-RD Scanning - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

Key features and details

  • Rabbit monoclonal [EP843(2)] to FGFR1 alpha (phospho Y654)
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant human FGFR1 alpha protein (Fc Chimera) (ab55758)

View more associated products

Overview

  • Product name

    Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)]
    See all FGFR1 alpha primary antibodies
  • Description

    Rabbit monoclonal [EP843(2)] to FGFR1 alpha (phospho Y654)
  • Host species

    Rabbit
  • Specificity

    Only detects FGFR1 alpha when phosphorylated at Tyrosine 654
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Tyrosine 654 of Human FGFR1 alpha.

  • Positive control

    • Constitutively active FGFR1
  • General notes

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.60 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP843(2)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • FGF
    • FGF Receptors
    • Signal Transduction
    • Growth Factors/Hormones
    • FGF
    • Cancer
    • Growth factors
    • FGF

Associated products

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human FGFR1 alpha protein (Fc Chimera) (ab55758)

Applications

Our Abpromise guarantee covers the use of ab134043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 130 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Relevance

      FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family and contains an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is widely expressed, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.
    • Cellular localization

      Cell Membrane
    • Database links

      • Entrez Gene: 14182 Mouse
      • SwissProt: P16092 Mouse
      • Unigene: 265716 Mouse
      • Alternative names

        • BFGFR antibody
        • CD331 antibody
        • CEK antibody
        • FGFBR antibody
        • FGFR1 antibody
        • FLG antibody
        • FLT2 antibody
        • FMS like tyrosine kinase 2 antibody
        • HBGFR antibody
        • KAL2 antibody
        • N SAM antibody
        • OGD antibody
        see all

      Images

      • Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
        Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
        All lanes : Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043) at 1/1000 dilution

        Lane 1 : Control
        Lane 2 : Constitutively active FGFR1 alpha
        Lane 3 : Kinase dead lysates from Xenopus oocytes

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-

        rabbit at 1/2000 dilution

        Predicted band size: 130 kDa

      • OI-RD Scanning - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
        OI-RD Scanning - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab134043? Please let us know so that we can cite the reference in this datasheet.

      ab134043 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Hello, I would like to know if this antibody is specific against the phosphorylated form of FGFR1 or if it cross-reacts with the phosphorylated forms of the other FGFRs. Also, I am interested in antibodies against pFGFR1 and pFGFR4 which do no cross-react with the other FGFRs. Could you please let me know if you have any of those? Thanks.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 23 2013

      Answer

      Thank you for contacting us.

      I have conducted a search through our catalogue for antibodies against pFGFR1 and pFGFR4 which do no cross-react with the other FGFRs.

      Ab59180 is specific against FGFR1 when it is phosphorilated at Tyr766. Similarly, ab59194 is specific against FGFR1 when it is phosphorilated at Tyr654, while ab52165 is specific against FGFR1 when it is phosphorilated at Tyr154.

      These three antibodies do not bind to non-phosphorilated proteins. If you would send me the accession numbers or the exact protein sequences of other FGFRs that you are concerned with cross reactivity, I can align them with our immunogen sequence for sequence homology and possible cross reactivity.

      We do have other alternatives (ab111124 and ab134043) against pFGFR1. However, these antibodies may also detect FGFR2, FGFR3 and FGFR4 phosphorilated at the equivalent sites. Although the antibodies have not been tested to determine if it detects these other endogenous forms, based on sequence homology reactivity would be expected.

      Unfortunately, all the https://www.abcam.com/Search?Keywords=FGFR4+&fReset=1&pt=1&source=TopSearch&btnSearch=Search are against non phosphorilated forms, so I am afraid they would not fit your requirements.

      Please do not hesitate to let me know whether you require a proforma, or any further assistance.

      Read More

      Abcam Scientific Support

      Answered on Jan 23 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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