RabMAb

Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

Submit a review Q&A (1)

Overview

  • Product name

    Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)]
    See all FGFR1 alpha primary antibodies

  • Description

    Rabbit monoclonal [EP843(2)] to FGFR1 alpha (phospho Y654)

  • Host species

    Rabbit

  • Specificity

    Only detects FGFR1 alpha when phosphorylated at Tyrosine 654

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Tyrosine 654 of Human FGFR1 alpha.

  • Positive control

    • Constitutively active FGFR1

  • General notes

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab134043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 130 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Relevance

      FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family and contains an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is widely expressed, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.

    • Cellular localization

      Cell Membrane

    • Database links

      • Alternative names

        • BFGFR antibody
        • CD331 antibody
        • CEK antibody
        • FGFBR antibody
        • FGFR1 antibody
        • FLG antibody
        • FLT2 antibody
        • FMS like tyrosine kinase 2 antibody
        • HBGFR antibody
        • KAL2 antibody
        • N SAM antibody
        • OGD antibody
        see all

      Images

      • Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
        Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
        All lanes : Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043) at 1/1000 dilution

        Lane 1 : Control
        Lane 2 : Constitutively active FGFR1 alpha
        Lane 3 : Kinase dead lysates from Xenopus oocytes

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-

        rabbit at 1/2000 dilution

        Predicted band size: 130 kDa

      • Other - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
        Other - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab134043 has not yet been referenced specifically in any publications.

      Publishing research using ab134043? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Hello, I would like to know if this antibody is specific against the phosphorylated form of FGFR1 or if it cross-reacts with the phosphorylated forms of the other FGFRs. Also, I am interested in antibodies against pFGFR1 and pFGFR4 which do no cross-react with the other FGFRs. Could you please let me know if you have any of those? Thanks.

      Read More
      Answer

      Thank you for contacting us.

      I have conducted a search through our catalogue for antibodies against pFGFR1 and pFGFR4 which do no cross-react with the other FGFRs.

      Ab59180 is specific against FGFR1 when it is phosphorilated at Tyr766. Similarly, ab59194 is specific against FGFR1 when it is phosphorilated at Tyr654, while ab52165 is specific against FGFR1 when it is phosphorilated at Tyr154.

      These three antibodies do not bind to non-phosphorilated proteins. If you would send me the accession numbers or the exact protein sequences of other FGFRs that you are concerned with cross reactivity, I can align them with our immunogen sequence for sequence homology and possible cross reactivity.

      We do have other alternatives (ab111124 and ab134043) against pFGFR1. However, these antibodies may also detect FGFR2, FGFR3 and FGFR4 phosphorilated at the equivalent sites. Although the antibodies have not been tested to determine if it detects these other endogenous forms, based on sequence homology reactivity would be expected.

      Unfortunately, all the https://www.abcam.com/Search?Keywords=FGFR4+&fReset=1&pt=1&source=TopSearch&btnSearch=Search are against non phosphorilated forms, so I am afraid they would not fit your requirements.

      Please do not hesitate to let me know whether you require a proforma, or any further assistance.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com