Product nameAnti-FGFR1 alpha (phospho Y654) antibody [EP843(2)]
See all FGFR1 alpha primary antibodies
DescriptionRabbit monoclonal [EP843(2)] to FGFR1 alpha (phospho Y654)
SpecificityOnly detects FGFR1 alpha when phosphorylated at Tyrosine 654
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Mouse
A phospho specific peptide corresponding to residues surrounding Tyrosine 654 of Human FGFR1 alpha.
- Constitutively active FGFR1
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.60 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 130 kDa.|
RelevanceFGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family and contains an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is widely expressed, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.
Cellular localizationCell Membrane
- BFGFR antibody
- CD331 antibody
- CEK antibody
All lanes : Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043) at 1/1000 dilution
Lane 1 : Control
Lane 2 : Constitutively active FGFR1 alpha
Lane 3 : Kinase dead lysates from Xenopus oocytes
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-
rabbit at 1/2000 dilution
Predicted band size: 130 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab134043 has not yet been referenced specifically in any publications.