Overview

  • Product name

    Anti-FGFR1 alpha (phospho Y766) antibody
    See all FGFR1 alpha primary antibodies
  • Description

    Rabbit polyclonal to FGFR1 alpha (phospho Y766)
  • Host species

    Rabbit
  • Specificity

    ab194527 detects endogenous level of FGFR1 only when phosphorylated at tyrosine 766.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human FGFR1 alpha (phospho Y766) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of tyrosine 766 (Q-E-Y(p)-L-D) derived from Human FGFR1.
    Database link: P11362

  • Positive control

    • HepG2 cell extracts, treated with EGF.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS

    PBS (without Mg2+and Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab194527 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 92 kDa.

Target

  • Relevance

    FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family and contains an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is widely expressed, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.
  • Cellular localization

    Cell Membrane
  • Database links

  • Alternative names

    • BFGFR antibody
    • CD331 antibody
    • CEK antibody
    • FGFBR antibody
    • FGFR1 antibody
    • FLG antibody
    • FLT2 antibody
    • FMS like tyrosine kinase 2 antibody
    • HBGFR antibody
    • KAL2 antibody
    • N SAM antibody
    • OGD antibody
    see all

Images

  • All lanes : Anti-FGFR1 alpha (phospho Y766) antibody (ab194527) at 1/500 dilution

    Lane 1 : HepG2 cell extracts, treated with EGF
    Lane 2 : HepG2 cell extracts, treated with EGF with antigen-specific peptide

References

ab194527 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab194527.
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