Overview

  • Product name

    Anti-FGFR1 antibody [EPR806Y]
    See all FGFR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR806Y] to FGFR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FGFR1 aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P11362
    (Peptide available as ab177436)

  • Positive control

    • WB: Wild-type HAP1, MCF-7 and SH-SY5Y cell lysate. IP: A-204 cell lysate ICC/IF: SH-SY5Y cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/500. Predicted molecular weight: 92 kDa.Can be blocked with FGFR1 peptide (ab177436).
IP 1/500.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Receptor for basic fibroblast growth factor. Receptor for FGF23 in the presence of KL (By similarity). A shorter form of the receptor could be a receptor for FGF1 (aFGF).
    • Tissue specificity

      Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.
    • Involvement in disease

      Defects in FGFR1 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly.
      Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
      Defects in FGFR1 are the cause of Kallmann syndrome type 2 (KAL2) [MIM:147950]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some cases, midline cranial anomalies (cleft lip/palate and imperfect fusion) are present and anosmia may be absent or inconspicuous.
      Defects in FGFR1 are the cause of osteoglophonic dysplasia (OGD) [MIM:166250]; also known as osteoglophonic dwarfism. OGD is characterized by craniosynostosis, prominent supraorbital ridge, and depressed nasal bridge, as well as by rhizomelic dwarfism and nonossifying bone lesions. Inheritance is autosomal dominant.
      Defects in FGFR1 are the cause of trigonocephaly non-syndromic (TRICEPH) [MIM:190440]; also known as metopic craniosynostosis. The term trigonocephaly describes the typical keel-shaped deformation of the forehead resulting from premature fusion of the frontal suture. Trigonocephaly may occur also as a part of a syndrome.
      Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Translocation t(8;13)(p11;q12) with ZMYM2. SCLL usually presents as lymphoblastic lymphoma in association with a myeloproliferative disorder, often accompanied by pronounced peripheral eosinophilia and/or prominent eosinophilic infiltrates in the affected bone marrow.
      Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1OP. Insertion ins(12;8)(p11;p11p22) with FGFR1OP2. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP2-FGFR1, FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.
      Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(8;9)(p12;q33) with CEP110. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion protein CEP110-FGFR1 is found in the cytoplasm, exhibits constitutive kinase activity and may be responsible for the transforming activity.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Binding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.
    • Cellular localization

      Membrane. Nucleus. Cytoplasm. Cytoplasmic vesicle
    • Information by UniProt
    • Database links

    • Alternative names

      • Basic fibroblast growth factor receptor 1 antibody
      • bFGF-R-1 antibody
      • BFGFR antibody
      • CD331 antibody
      • CEK antibody
      • FGFBR antibody
      • FGFR 1 antibody
      • FGFR-1 antibody
      • FGFR1 antibody
      • FGFR1/PLAG1 fusion antibody
      • FGFR1_HUMAN antibody
      • fibroblast growth factor receptor 1 antibody
      • FLG antibody
      • FLT-2 antibody
      • FLT2 antibody
      • Fms-like gene antibody
      • Fms-like tyrosine kinase 2 antibody
      • fms-related tyrosine kinase 2 antibody
      • HBGFR antibody
      • heparin-binding growth factor receptor antibody
      • HH2 antibody
      • HRTFDS antibody
      • hydroxyaryl-protein kinase antibody
      • KAL2 antibody
      • N-SAM antibody
      • OGD antibody
      • Proto-oncogene c-Fgr antibody
      see all

    Images

    • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: FGFR1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: MCF7 whole cell lysate (20 µg)
      Lane 4: SH-SY5Y whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab76464 observed at 140 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab76464 was shown to specifically react with FGFR1 when FGFR1 knockout samples were used. Wild-type and FGFR1 knockout samples were subjected to SDS-PAGE.  ab76464 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/mL and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

       

    • Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling FGFR1  with  at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in SH-SY5Y cell line is observed. Ab195889  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)  was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    • ab76464 (purified) at 1/500 immunoprecipitating FGFR1 in 10 μg A-204 (Human muscle rhabdomyosarcoma)whole cell lysate (Lanes 1 and 2, observed at 145 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730) instead of ab76464 in A-204 whole cell lysate. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution.

      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

       

    • Anti-FGFR1 antibody [EPR806Y] (ab76464) at 1/500 dilution (purified) + SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 92 kDa
      Observed band size: 145 kDa
      why is the actual band size different from the predicted?



      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Anti-FGFR1 antibody [EPR806Y] (ab76464) at 1/500 dilution (unpurified) + SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 92 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Jenks AD  et al. Primary Cilia Mediate Diverse Kinase Inhibitor Resistance Mechanisms in Cancer. Cell Rep 23:3042-3055 (2018). WB . Read more (PubMed: 29874589) »
    • Hu T  et al. FGFR1 fusion kinase regulation of MYC expression drives development of stem cell leukemia/lymphoma syndrome. Leukemia 32:2363-2373 (2018). Read more (PubMed: 29720732) »
    See all 13 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    ELISA
    Sample
    Human Recombinant protein (Extra Cellular Domain - Biotin Conjugated)
    Specification
    Extra Cellular Domain - Biotin Conjugated
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Type
    Sandwich (Capture)

    Abcam user community

    Verified customer

    Submitted Nov 13 2018

    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (Adipose Tissue)
    Gel Running Conditions
    Reduced Denaturing (7.5)
    Loading amount
    20 µg
    Specification
    Adipose Tissue
    Blocking step
    Licor Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Mar 13 2018

    Answer

    Thank you for contacting us.

    Please search in literature for evidence about the negativity of cell lines. NCI-H1703 does express FGFR3.

    http://rcgdb.bioinf.uni-sb.de/MutomeWeb/SimpleSampleQuery?query=NCI-H1703

    http://www.ebi.ac.uk/gxa/experiment/E-MTAB-37/ENSG00000068078

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up