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    fgfr1-antibody-epr806y-ab76464.pdf

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Cardiovascular Angiogenesis Growth Factors FGF FGF Receptors
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-FGFR1 antibody [EPR806Y] (ab76464)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (25)

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Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464)
  • Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody [EPR806Y] (ab76464)
  • Immunoprecipitation - Anti-FGFR1 antibody [EPR806Y] (ab76464)
  • Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464)
  • Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464)
  • Anti-FGFR1 antibody [EPR806Y] (ab76464)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR806Y] to FGFR1
  • Suitable for: ICC/IF, WB, IP
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-FGFR1 antibody [EPR806Y]
    See all FGFR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR806Y] to FGFR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FGFR1 aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P11362
    (Peptide available as ab177436)

  • Positive control

    • WB: Wild-type HAP1, MCF-7 and SH-SY5Y cell lysate. IP: A-204 cell lysate ICC/IF: SH-SY5Y cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR806Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • FGF
    • FGF Receptors
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • FGF
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Growth factors
    • FGF
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free (ab206382)
    • Anti-FGFR1 antibody [EPR806Y] - BSA and Azide free (ab271861)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human FGFR1 protein (Active) (ab168696)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
WB
1/500. Predicted molecular weight: 92 kDa.
IP
1/190.
Notes
ICC/IF
1/100.
WB
1/500. Predicted molecular weight: 92 kDa.
IP
1/190.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Receptor for basic fibroblast growth factor. Receptor for FGF23 in the presence of KL (By similarity). A shorter form of the receptor could be a receptor for FGF1 (aFGF).
  • Tissue specificity

    Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.
  • Involvement in disease

    Defects in FGFR1 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly.
    Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
    Defects in FGFR1 are the cause of Kallmann syndrome type 2 (KAL2) [MIM:147950]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some cases, midline cranial anomalies (cleft lip/palate and imperfect fusion) are present and anosmia may be absent or inconspicuous.
    Defects in FGFR1 are the cause of osteoglophonic dysplasia (OGD) [MIM:166250]; also known as osteoglophonic dwarfism. OGD is characterized by craniosynostosis, prominent supraorbital ridge, and depressed nasal bridge, as well as by rhizomelic dwarfism and nonossifying bone lesions. Inheritance is autosomal dominant.
    Defects in FGFR1 are the cause of trigonocephaly non-syndromic (TRICEPH) [MIM:190440]; also known as metopic craniosynostosis. The term trigonocephaly describes the typical keel-shaped deformation of the forehead resulting from premature fusion of the frontal suture. Trigonocephaly may occur also as a part of a syndrome.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Translocation t(8;13)(p11;q12) with ZMYM2. SCLL usually presents as lymphoblastic lymphoma in association with a myeloproliferative disorder, often accompanied by pronounced peripheral eosinophilia and/or prominent eosinophilic infiltrates in the affected bone marrow.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1OP. Insertion ins(12;8)(p11;p11p22) with FGFR1OP2. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP2-FGFR1, FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(8;9)(p12;q33) with CEP110. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion protein CEP110-FGFR1 is found in the cytoplasm, exhibits constitutive kinase activity and may be responsible for the transforming activity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Binding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.
  • Cellular localization

    Membrane. Nucleus. Cytoplasm. Cytoplasmic vesicle
  • Target information above from: UniProt accession P11362 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2260 Human
    • Omim: 136350 Human
    • SwissProt: P11362 Human
    • Unigene: 264887 Human
    • Alternative names

      • Basic fibroblast growth factor receptor 1 antibody
      • bFGF-R-1 antibody
      • BFGFR antibody
      • CD331 antibody
      • CEK antibody
      • FGFBR antibody
      • FGFR 1 antibody
      • FGFR-1 antibody
      • FGFR1 antibody
      • FGFR1/PLAG1 fusion antibody
      • FGFR1_HUMAN antibody
      • fibroblast growth factor receptor 1 antibody
      • FLG antibody
      • FLT-2 antibody
      • FLT2 antibody
      • Fms-like gene antibody
      • Fms-like tyrosine kinase 2 antibody
      • fms-related tyrosine kinase 2 antibody
      • HBGFR antibody
      • heparin-binding growth factor receptor antibody
      • HH2 antibody
      • HRTFDS antibody
      • hydroxyaryl-protein kinase antibody
      • KAL2 antibody
      • N-SAM antibody
      • OGD antibody
      • Proto-oncogene c-Fgr antibody
      see all

    Images

    • Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464)
      Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: FGFR1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: MCF7 whole cell lysate (20 µg)
      Lane 4: SH-SY5Y whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab76464 observed at 140 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab76464 was shown to specifically react with FGFR1 when FGFR1 knockout samples were used. Wild-type and FGFR1 knockout samples were subjected to SDS-PAGE.  ab76464 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/mL and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody [EPR806Y] (ab76464)
      Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody [EPR806Y] (ab76464)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling FGFR1  with  at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in SH-SY5Y cell line is observed. Ab195889  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)  was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    • Immunoprecipitation - Anti-FGFR1 antibody [EPR806Y] (ab76464)
      Immunoprecipitation - Anti-FGFR1 antibody [EPR806Y] (ab76464)

      ab76464 (purified) at 1/190 immunoprecipitating FGFR1 in 10 μg A-204 (Human muscle rhabdomyosarcoma)whole cell lysate (Lanes 1 and 2, observed at 145 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730) instead of ab76464 in A-204 whole cell lysate. For western blotting, ab76464 at 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

       

    • Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464)
      Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464)
      Anti-FGFR1 antibody [EPR806Y] (ab76464) at 1/500 dilution (purified) + SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 92 kDa
      Observed band size: 145 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464)
      Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464)
      Anti-FGFR1 antibody [EPR806Y] (ab76464) at 1/500 dilution (unpurified) + SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 92 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?

    • Anti-FGFR1 antibody [EPR806Y] (ab76464)
      Anti-FGFR1 antibody [EPR806Y] (ab76464)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (25)

    Publishing research using ab76464? Please let us know so that we can cite the reference in this datasheet.

    ab76464 has been referenced in 25 publications.

    • Yang L  et al. MiniPDX-guided postoperative anticancer treatment can effectively prolong the survival of patients with hepatocellular carcinoma. Cancer Chemother Pharmacol 87:125-134 (2021). PubMed: 33141330
    • Li J  et al. A pentapeptide enabled AL3810 liposome-based glioma-targeted therapy with immune opsonic effect attenuated. Acta Pharm Sin B 11:283-299 (2021). PubMed: 33532193
    • Yun Z  et al. Long non-coding RNA CERS6-AS1 facilitates the oncogenicity of pancreatic ductal adenocarcinoma by regulating the microRNA-15a-5p/FGFR1 axis. Aging (Albany NY) 13:6041-6054 (2021). PubMed: 33581689
    • Wang X  et al. LncRNA PVT1 Facilitates Proliferation, Migration and Invasion of NSCLC Cells via miR-551b/FGFR1 Axis. Onco Targets Ther 14:3555-3565 (2021). PubMed: 34113122
    • Wang C  et al. Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2. Nat Commun 11:3162 (2020). PubMed: 32572027
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews

    ELISA abreview for Anti-FGFR1 antibody [EPR806Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Recombinant protein (Extra Cellular Domain - Biotin Conjugated)
    Specification
    Extra Cellular Domain - Biotin Conjugated
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Type
    Sandwich (Capture)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 13 2018

    Western blot abreview for Anti-FGFR1 antibody [EPR806Y]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (Adipose Tissue)
    Gel Running Conditions
    Reduced Denaturing (7.5)
    Loading amount
    20 µg
    Specification
    Adipose Tissue
    Blocking step
    Licor Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 13 2018

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