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  1. Link

    fgfr1-oncogene-partner-antibody-epr9929-ab156013.pdf

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RecombinantRabMAb

Recombinant Anti-FGFR1 Oncogene Partner antibody [EPR9929] (ab156013)

  • Datasheet
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Western blot - Anti-FGFR1 Oncogene Partner antibody [EPR9929] (ab156013)
  • Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 Oncogene Partner antibody [EPR9929] (ab156013)
  • Anti-FGFR1 Oncogene Partner antibody [EPR9929] (ab156013)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9929] to FGFR1 Oncogene Partner
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Recombinant Human FGFR1 Oncogene Partner protein (ab126678)
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Overview

  • Product name

    Anti-FGFR1 Oncogene Partner antibody [EPR9929]
    See all FGFR1 Oncogene Partner primary antibodies
  • Description

    Rabbit monoclonal [EPR9929] to FGFR1 Oncogene Partner
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, 293T and Saos-2 cell lysates; HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9929
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Centrosome
    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Associated products

  • Alternative Versions

    • Anti-FGFR1 Oncogene Partner antibody [EPR9929] - BSA and Azide free (ab249252)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human FGFR1 Oncogene Partner protein (ab126678)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab156013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Required for anchoring microtubules to the centrosomes.
  • Tissue specificity

    Ubiquitous. Highly expressed in heart, liver, muscle, kidney, intestine, colon, adrenal gland, prostate, testis, and pancreas.
  • Involvement in disease

    Note=A chromosomal aberration involving FGFR1OP may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.
  • Sequence similarities

    Belongs to the FGFR1OP family.
    Contains 1 LisH domain.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Associated with gamma-tubulin.
  • Target information above from: UniProt accession O95684 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 11116 Human
    • Entrez Gene: 75296 Mouse
    • Entrez Gene: 365103 Rat
    • Entrez Gene: 683722 Rat
    • Omim: 605392 Human
    • SwissProt: O95684 Human
    • SwissProt: Q66JX5 Mouse
    • SwissProt: Q4V7C1 Rat
    • Unigene: 487175 Human
    • Unigene: 227250 Mouse
    • Unigene: 166829 Rat
    see all
  • Alternative names

    • FGFR1 oncogene partner antibody
    • FGFR1OP antibody
    • Fibroblast growth factor receptor 1 oncogene partner antibody
    • FOP antibody
    • FR1OP_HUMAN antibody
    • OTTHUMP00000017612 antibody
    • OTTHUMP00000017613 antibody
    see all

Images

  • Western blot - Anti-FGFR1 Oncogene Partner antibody [EPR9929] (ab156013)
    Western blot - Anti-FGFR1 Oncogene Partner antibody [EPR9929] (ab156013)
    All lanes : Anti-FGFR1 Oncogene Partner antibody [EPR9929] (ab156013) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Saos-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 43 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 Oncogene Partner antibody [EPR9929] (ab156013)
    Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 Oncogene Partner antibody [EPR9929] (ab156013)
    Immunofluorescent analysis of HeLa cells labeling FGFR1 Oncogene Partner with ab156013 at 1/100 dilution.
  • Anti-FGFR1 Oncogene Partner antibody [EPR9929] (ab156013)
    Anti-FGFR1 Oncogene Partner antibody [EPR9929] (ab156013)

Protocols

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab156013? Please let us know so that we can cite the reference in this datasheet.

ab156013 has been referenced in 1 publication.

  • Jiang H  et al. FOP Negatively Regulates Ciliogenesis and Promotes Cell Cycle Re-entry by Facilitating Primary Cilia Disassembly. Front Cell Dev Biol 8:590449 (2020). PubMed: 33304902

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