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    fgfr1-phospho-y653-antibody-epr843n-ab173305.pdf

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Cardiovascular Angiogenesis Growth Factors FGF FGF Receptors
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RecombinantRabMAb

Recombinant Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] (ab173305)

  • Datasheet
Reviews (1) Submit a question References (7)

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Western blot - Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] (ab173305)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] (ab173305)
  • Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] (ab173305)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR843(N)] to FGFR1 (phospho Y653)
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-FGFR1 (phospho Y653) antibody [EPR843(N)]
    See all FGFR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR843(N)] to FGFR1 (phospho Y653)
  • Host species

    Rabbit
  • Specificity

    Stimulation may be required to allow detection of the phosphorylated protein.  Please see images below for recommended treatment conditions and positive controls.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human FGFR1 aa 600-700 (phospho Y653) (Cysteine residue). The exact sequence is proprietary.
    Database link: P11362
    (Peptide available as ab205614)

  • Positive control

    • WB: NIH/3T3 cell lysate treated with pervanadate. IHC-P: Human glioma tissue.
  • General notes

    This antibody was developed as part of a collaboration between Epitomics and Ira Daar at the National Cancer Institute, NIH.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR843(N)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • FGF
    • FGF Receptors
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • FGF
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Growth factors
    • FGF
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] - BSA and Azide free (ab213609)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human FGFR1 protein (Active) (ab168696)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab173305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 92 kDa.
IHC-P (1)
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 92 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC or IP.

Target

  • Function

    Receptor for basic fibroblast growth factor. Receptor for FGF23 in the presence of KL (By similarity). A shorter form of the receptor could be a receptor for FGF1 (aFGF).
  • Tissue specificity

    Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.
  • Involvement in disease

    Defects in FGFR1 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly.
    Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
    Defects in FGFR1 are the cause of Kallmann syndrome type 2 (KAL2) [MIM:147950]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some cases, midline cranial anomalies (cleft lip/palate and imperfect fusion) are present and anosmia may be absent or inconspicuous.
    Defects in FGFR1 are the cause of osteoglophonic dysplasia (OGD) [MIM:166250]; also known as osteoglophonic dwarfism. OGD is characterized by craniosynostosis, prominent supraorbital ridge, and depressed nasal bridge, as well as by rhizomelic dwarfism and nonossifying bone lesions. Inheritance is autosomal dominant.
    Defects in FGFR1 are the cause of trigonocephaly non-syndromic (TRICEPH) [MIM:190440]; also known as metopic craniosynostosis. The term trigonocephaly describes the typical keel-shaped deformation of the forehead resulting from premature fusion of the frontal suture. Trigonocephaly may occur also as a part of a syndrome.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Translocation t(8;13)(p11;q12) with ZMYM2. SCLL usually presents as lymphoblastic lymphoma in association with a myeloproliferative disorder, often accompanied by pronounced peripheral eosinophilia and/or prominent eosinophilic infiltrates in the affected bone marrow.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1OP. Insertion ins(12;8)(p11;p11p22) with FGFR1OP2. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP2-FGFR1, FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(8;9)(p12;q33) with CEP110. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion protein CEP110-FGFR1 is found in the cytoplasm, exhibits constitutive kinase activity and may be responsible for the transforming activity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Binding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.
  • Cellular localization

    Membrane. Nucleus. Cytoplasm. Cytoplasmic vesicle
  • Target information above from: UniProt accession P11362 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2260 Human
    • Entrez Gene: 14182 Mouse
    • Entrez Gene: 79114 Rat
    • Omim: 136350 Human
    • SwissProt: P11362 Human
    • SwissProt: P16092 Mouse
    • SwissProt: Q04589 Rat
    • Unigene: 264887 Human
    • Unigene: 265716 Mouse
    • Unigene: 207203 Rat
    • Unigene: 9797 Rat
    see all
  • Alternative names

    • Basic fibroblast growth factor receptor 1 antibody
    • bFGF-R-1 antibody
    • BFGFR antibody
    • CD331 antibody
    • CEK antibody
    • FGFBR antibody
    • FGFR 1 antibody
    • FGFR-1 antibody
    • FGFR1 antibody
    • FGFR1/PLAG1 fusion antibody
    • FGFR1_HUMAN antibody
    • fibroblast growth factor receptor 1 antibody
    • FLG antibody
    • FLT-2 antibody
    • FLT2 antibody
    • Fms-like gene antibody
    • Fms-like tyrosine kinase 2 antibody
    • fms-related tyrosine kinase 2 antibody
    • HBGFR antibody
    • heparin-binding growth factor receptor antibody
    • HH2 antibody
    • HRTFDS antibody
    • hydroxyaryl-protein kinase antibody
    • KAL2 antibody
    • N-SAM antibody
    • OGD antibody
    • Proto-oncogene c-Fgr antibody
    see all

Images

  • Western blot - Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] (ab173305)
    Western blot - Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] (ab173305)
    All lanes : Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] (ab173305) at 1/1000 dilution

    Lane 1 : Untreated NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
    Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 92 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] (ab173305)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] (ab173305)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling FGFR1 (phospho Y653) with ab173305 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] (ab173305)
    Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] (ab173305)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (7)

Publishing research using ab173305? Please let us know so that we can cite the reference in this datasheet.

ab173305 has been referenced in 7 publications.

  • Homer-Bouthiette C  et al. Gait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice. Sci Rep 11:11005 (2021). PubMed: 34040128
  • Bhattacharyya S  et al. Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer. J Exp Med 217:N/A (2020). PubMed: 32434218
  • Li L  et al. Brivanib, a multitargeted small-molecule tyrosine kinase inhibitor, suppresses laser-induced CNV in a mouse model of neovascular AMD. J Cell Physiol 235:1259-1273 (2020). PubMed: 31270802
  • Zhen C  et al. MicroRNA-652 inhibits the biological characteristics of esophageal squamous cell carcinoma by directly targeting fibroblast growth factor receptor 1. Exp Ther Med 18:4473-4480 (2019). PubMed: 31777550
  • Liu J  et al. Peptide-based NTA(Ni)-nanodiscs for studying membrane enhanced FGFR1 kinase activities. PeerJ 7:e7234 (2019). PubMed: 31372315
  • Kang J  et al. A Selective FGFR inhibitor AZD4547 suppresses RANKL/M-CSF/OPG-dependent ostoclastogenesis and breast cancer growth in the metastatic bone microenvironment. Sci Rep 9:8726 (2019). PubMed: 31217507
  • Seo HR  et al. Intrinsic FGF2 and FGF5 promotes angiogenesis of human aortic endothelial cells in 3D microfluidic angiogenesis system. Sci Rep 6:28832 (2016). WB ; Human . PubMed: 27357248

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FGFR1 (phospho Y653) antibody [EPR843(N)]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tumor generated by s.c. injection of mouse NIH3T3)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
tumor generated by s.c. injection of mouse NIH3T3
Fixative
Paraformaldehyde
Read More

Oreste Segatto

Verified customer

Submitted Jan 06 2018

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