Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FGFR1 (phospho Y653) antibody [EPR843(N)]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tumor generated by s.c. injection of mouse NIH3T3)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
tumor generated by s.c. injection of mouse NIH3T3
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Bond Polymer Refine Detection
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Immunoreactivity was revealed using Bond Polymer Refine Detection, a biotin-free, polymeric horseradish peroxidase (HRP)-linker antibody conjugate system on an automated autostainer(BondTM-III, Leica Biosystem, Milan, Italy) according to the manufacturer's instructions. Our data show that ab173305 detects tyrosine-phosphorylated human FGFR2 in the context of an FGFR2-TACC3 fusion protein.

Oreste Segatto

Verified customer

Submitted Jan 06 2018

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