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Immunohistochemistry (Frozen sections) abreview for Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] - BSA and Azide free

Good
Abreviews
Go to datasheet (ab213609)
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (HAIR FOLLICLE)
Permeabilization
Yes - PBST 0.05% (teewn)
Specification
HAIR FOLLICLE
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: HISS/PBS 10%
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Rhodamine (TRITC) Aff
Host species: Donkey
Clonality: Polyclonal
Conjugation: TRITC

Additional data

Additional Notes
use fresh frozen section and do not do antigen retrieval
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Mar 09 2018