Our Abpromise guarantee covers the use of ab10647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IHC-P: Use at an assay dependent dilution, this concentration is determined by indirect immunoperoxidase staining of trypsin or protease-digested human and animal tissue sections.
    IP: 1/2000. This concentration is determined by immunoprecipitation using a whole lysate of transfected cells expressing recombinant human FGFR2.
    WB: 1/2000. This concentration is determined by immunoblotting using a whole extract of transfected cells expressing recombinant human FGFR2. Predicted molecular weight: 110 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Receptor for acidic and basic fibroblast growth factors.
    • Involvement in disease

      Defects in FGFR2 are the cause of Crouzon syndrome (CS) [MIM:123500]; also called craniofacial dysostosis type I (CFD1). CS is an autosomal dominant syndrome characterized by craniosynostosis (premature fusion of the skull sutures), hypertelorism, exophthalmos and external strabismus, parrot-beaked nose, short upper lip, hypoplastic maxilla, and a relative mandibular prognathism.
      Defects in FGFR2 are a cause of Jackson-Weiss syndrome (JWS) [MIM:123150]. JWS is an autosomal dominant craniosynostosis syndrome characterized by craniofacial abnormalities and abnormality of the feet: broad great toes with medial deviation and tarsal-metatarsal coalescence.
      Defects in FGFR2 are a cause of Apert syndrome (APRS) [MIM:101200]; also known as acrocephalosyndactyly type 1 (ACS1). APRS is a syndrome characterized by facio-cranio-synostosis, osseous and membranous syndactyly of the four extremities, and midface hypoplasia. The craniosynostosis is bicoronal and results in acrocephaly of brachysphenocephalic type. Syndactyly of the fingers and toes may be total (mitten hands and sock feet) or partial affecting the second, third, and fourth digits. Intellectual deficit is frequent and often severe, usually being associated with cerebral malformations.
      Defects in FGFR2 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly. Three subtypes of Pfeiffer syndrome have been described: mild autosomal dominant form (type 1); cloverleaf skull, elbow ankylosis, early death, sporadic (type 2); craniosynostosis, early demise, sporadic (type 3).
      Defects in FGFR2 are the cause of Beare-Stevenson cutis gyrata syndrome (BSCGS) [MIM:123790]. BSCGS is an autosomal dominant condition is characterized by the furrowed skin disorder of cutis gyrata, acanthosis nigricans, craniosynostosis, craniofacial dysmorphism, digital anomalies, umbilical and anogenital abnormalities and early death.
      Defects in FGFR2 are the cause of familial scaphocephaly syndrome (FSPC) [MIM:609579]; also known as scaphocephaly with maxillary retrusion and mental retardation. FSPC is an autosomal dominant craniosynostosis syndrome characterized by scaphocephaly, macrocephaly, hypertelorism, maxillary retrusion, and mild intellectual disability. Scaphocephaly is the most common of the craniosynostosis conditions and is characterized by a long, narrow head. It is due to premature fusion of the sagittal suture or from external deformation.
      Defects in FGFR2 are a cause of lacrimo-auriculo-dento-digital syndrome (LADDS) [MIM:149730]; also known as Levy-Hollister syndrome. LADDS is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. LADDS is an autosomal dominant syndrome characterized by aplastic/hypoplastic lacrimal and salivary glands and ducts, cup-shaped ears, hearing loss, hypodontia and enamel hypoplasia, and distal limb segments anomalies. In addition to these cardinal features, facial dysmorphism, malformations of the kidney and respiratory system and abnormal genitalia have been reported. Craniosynostosis and severe syndactyly are not observed.
      Defects in FGFR2 are the cause of Antley-Bixler syndrome (ABS) [MIM:207410]. ABS is a multiple congenital anomaly syndrome characterized by craniosynostosis, radiohumeral synostosis, midface hypoplasia, malformed ears, arachnodactyly and multiple joint contractures. ABS is a heterogeneous disorder and occurs with and without abnormal genitalia in both sexes.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • bacteria-expressed kinase antibody
      • BBDS antibody
      • BEK antibody
      • BEK fibroblast growth factor receptor antibody
      • BFR1 antibody
      • CD332 antibody
      • CD332 antigen antibody
      • CEK3 antibody
      • CFD1 antibody
      • Craniofacial dysostosis 1 antibody
      • ECT1 antibody
      • FGF receptor antibody
      • FGFR 2 antibody
      • FGFR-2 antibody
      • Fgfr2 antibody
      • FGFR2_HUMAN antibody
      • Fibroblast growth factor receptor 2 antibody
      • Hydroxyaryl protein kinase antibody
      • Jackson Weiss syndrome antibody
      • JWS antibody
      • K SAM antibody
      • K-sam antibody
      • Keratinocyte growth factor receptor 2 antibody
      • Keratinocyte growth factor receptor antibody
      • KGFR antibody
      • KSAM antibody
      • protein tyrosine kinase, receptor like 14 antibody
      • soluble FGFR4 variant 4 antibody
      • TK14 antibody
      • TK25 antibody
      see all


    This product has been referenced in:

    • Vesterlund L  et al. Co-localization of neural cell adhesion molecule and fibroblast growth factor receptor 2 in early embryo development. Int J Dev Biol 55:313-9 (2011). Read more (PubMed: 21710437) »
    • Spence JR  et al. Retina regeneration in the chick embryo is not induced by spontaneous Mitf downregulation but requires FGF/FGFR/MEK/Erk dependent upregulation of Pax6. Mol Vis 13:57-65 (2007). Read more (PubMed: 17277739) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A


    Thank you for your enquiry. I have confirmed that the discrepancy is indeed a typographical error. I have updated the datasheet with the correct sequence. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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    Thank you for your enquiry. Further to correspondence with the source of this antibody unfortunately I have been unable to determine whether the multiple bands that you have been observing is typical of any of these antibodies. As a general rule if a customer has high background on their blot we recommend that they try changing the blocking buffer to BSA, increasing the stringency and duration of the wash conditions and decreasing the concentration of the antibody. Should you continue to obtain these extraneous bands I would greatly appreciate it if you complete our technical questionnaire by clicking on the links below. This will greatly help our technical team determine the steps that you have taken to optimise this antibody. It would be most useful if you could additionally provide us with the LOT numbers and representative images of the blots. I look forward to hearing from you. ab5481 https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=5481&mode=questionaire ab10646 https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=10646&mode=questionaire ab10647 https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=10647&mode=questionaire ab10649 https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=10649&mode=questionaire

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    I was able to find out what protocol was used on transfected 293T cells expressing recombinant human FGFR2: 1. Extract: 0.5 plate/slab of transfected 293T cells expressing recombinant human FGFR-2. 7-10% gel. The proteins were run on a reducing gel. 2. Semi dry transfer for 90 minutes at room temperature. 3. Blocking solution: 10% non fat dry milk or 10% BSA for 1-2 hours at room temperature. 4. Wash and Dilution buffer: 1% BSA in PBS-T. 5. Primary antibody: this product at 1:2,000-1:4,000 dilutions. Incubation for 120 minutes at room temperature. 6. Secondary antibody: Alk Phos anti rabbit IgG at 1:5,000 dilution. Incubation for 60 minutes at room temperature. I hope this piece of extra information helps you. 7. Substrate: BCIP/NBT. This product was tested in immunohistology on rat kidney and mouse tongue and found positive with good results.

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    Thank you very much for taking the time to send me the photograph of your blots and for the extra information, it is very useful. The source has no reported problems with this antibody. The size range of FGFR2 receptor should be between 115 and 125KDa. But the numerous isoforms can be glycosylated and/or cleaved, and oligomerized, so it is very possible that you are detecting a large number of isoforms. Here are some references regarding the various isoforms: -Miki et al, PNAS USA, 89,p246 (1992) -Johnson et al, Mol cell biol, 11, p4627 (1991) -Levi et al, PNAS USA, 91, p9170 (1996) -Yayon et al, EMBOJ, 5, p1885 (1992) Please let me know if this information helps and if you need further advice.

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    Thank you very much for your enquiry. Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

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