Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab10648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 110 kDa. 1/2000 this dilution is determined by blotting using a whole extract of transfected cells expressing recombinant human FGFR2. Predicted molecular weight: 110 kDa.
IP 1/1000. 1/1000, this dilution is determined by immunoprecipitation using a whole lysate of transfected cells expressing recombinant human FGFR2.
IHC-P 1/1000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. The recommended enzyme is trypsin.

Target

  • Function
    Receptor for acidic and basic fibroblast growth factors.
  • Involvement in disease
    Defects in FGFR2 are the cause of Crouzon syndrome (CS) [MIM:123500]; also called craniofacial dysostosis type I (CFD1). CS is an autosomal dominant syndrome characterized by craniosynostosis (premature fusion of the skull sutures), hypertelorism, exophthalmos and external strabismus, parrot-beaked nose, short upper lip, hypoplastic maxilla, and a relative mandibular prognathism.
    Defects in FGFR2 are a cause of Jackson-Weiss syndrome (JWS) [MIM:123150]. JWS is an autosomal dominant craniosynostosis syndrome characterized by craniofacial abnormalities and abnormality of the feet: broad great toes with medial deviation and tarsal-metatarsal coalescence.
    Defects in FGFR2 are a cause of Apert syndrome (APRS) [MIM:101200]; also known as acrocephalosyndactyly type 1 (ACS1). APRS is a syndrome characterized by facio-cranio-synostosis, osseous and membranous syndactyly of the four extremities, and midface hypoplasia. The craniosynostosis is bicoronal and results in acrocephaly of brachysphenocephalic type. Syndactyly of the fingers and toes may be total (mitten hands and sock feet) or partial affecting the second, third, and fourth digits. Intellectual deficit is frequent and often severe, usually being associated with cerebral malformations.
    Defects in FGFR2 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly. Three subtypes of Pfeiffer syndrome have been described: mild autosomal dominant form (type 1); cloverleaf skull, elbow ankylosis, early death, sporadic (type 2); craniosynostosis, early demise, sporadic (type 3).
    Defects in FGFR2 are the cause of Beare-Stevenson cutis gyrata syndrome (BSCGS) [MIM:123790]. BSCGS is an autosomal dominant condition is characterized by the furrowed skin disorder of cutis gyrata, acanthosis nigricans, craniosynostosis, craniofacial dysmorphism, digital anomalies, umbilical and anogenital abnormalities and early death.
    Defects in FGFR2 are the cause of familial scaphocephaly syndrome (FSPC) [MIM:609579]; also known as scaphocephaly with maxillary retrusion and mental retardation. FSPC is an autosomal dominant craniosynostosis syndrome characterized by scaphocephaly, macrocephaly, hypertelorism, maxillary retrusion, and mild intellectual disability. Scaphocephaly is the most common of the craniosynostosis conditions and is characterized by a long, narrow head. It is due to premature fusion of the sagittal suture or from external deformation.
    Defects in FGFR2 are a cause of lacrimo-auriculo-dento-digital syndrome (LADDS) [MIM:149730]; also known as Levy-Hollister syndrome. LADDS is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. LADDS is an autosomal dominant syndrome characterized by aplastic/hypoplastic lacrimal and salivary glands and ducts, cup-shaped ears, hearing loss, hypodontia and enamel hypoplasia, and distal limb segments anomalies. In addition to these cardinal features, facial dysmorphism, malformations of the kidney and respiratory system and abnormal genitalia have been reported. Craniosynostosis and severe syndactyly are not observed.
    Defects in FGFR2 are the cause of Antley-Bixler syndrome (ABS) [MIM:207410]. ABS is a multiple congenital anomaly syndrome characterized by craniosynostosis, radiohumeral synostosis, midface hypoplasia, malformed ears, arachnodactyly and multiple joint contractures. ABS is a heterogeneous disorder and occurs with and without abnormal genitalia in both sexes.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • bacteria-expressed kinase antibody
    • BBDS antibody
    • BEK antibody
    • BEK fibroblast growth factor receptor antibody
    • BFR1 antibody
    • CD332 antibody
    • CD332 antigen antibody
    • CEK3 antibody
    • CFD1 antibody
    • Craniofacial dysostosis 1 antibody
    • ECT1 antibody
    • FGF receptor antibody
    • FGFR 2 antibody
    • FGFR-2 antibody
    • Fgfr2 antibody
    • FGFR2_HUMAN antibody
    • Fibroblast growth factor receptor 2 antibody
    • Hydroxyaryl protein kinase antibody
    • Jackson Weiss syndrome antibody
    • JWS antibody
    • K SAM antibody
    • K-sam antibody
    • Keratinocyte growth factor receptor 2 antibody
    • Keratinocyte growth factor receptor antibody
    • KGFR antibody
    • KSAM antibody
    • protein tyrosine kinase, receptor like 14 antibody
    • soluble FGFR4 variant 4 antibody
    • TK14 antibody
    • TK25 antibody
    see all

Images

  • ab10648 staining FGFR2 in the epithelium (top) and transition zone (bottom) of Embryonic Mouse eye tissue sections by Immunohistochemistry ((IHC) paraffin-embedded sections). Tissue was fixed, embedded in paraffin and sectioned. Sections were trypsinized for 40 minutes at room temperature in a humidified chamber, washed in PBS, and then incubated for 1 hour with a 0.5% Triton X-100 and 0.3 M glycine in PBS in a humidified chamber. After antigen retrieval, sections were blocked in 0.5% nonfat dry milk, 10% horse serum, and 0.2% Triton X-100 diluted in PBS for 3 hours at room temperature. An Alexa Fluor®568-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded analysis of hb/hb and control mouse embryo (P5) tissue sections labelling FGFR2 with ab10648. Samples were fixed overnight at 4°C in 10% neutral-buffered formalin, embedded in paraffin and cut into 8µm sections.

    At this stage, FGFR2 is located in hair cells (black arrowheads) and tectorial membrane (red arrowhead). No significant differences in the Fgfr2 protein levels are detected in hb/hb mutants compared to controls. Scale bar: 10µm.

References

This product has been referenced in:
See all 23 Publications for this product

Customer reviews and Q&As

1-10 of 17 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse heads)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer pH 7.8
Permeabilization
Yes - PBST
Specification
mouse heads
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 24 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 30 2017

Answer

The immunogen of this antibody is from aa362 - 374 so this antibody should bind to isoform 3 (FGF R2 IIIb) as well. The immunogen is shared between different isoform so please note this product cannot be used to distinguish the isoforms of FGFR2.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Melanoma cancer)
Loading amount
30 µg
Specification
Melanoma cancer
Treatment
5gy Irradiation
Gel Running Conditions
Non-reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C

Ms. Seontae Kim

Verified customer

Submitted Jan 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Skin section)
Specification
Skin section
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 03 2012

Answer

Thank you for contacting us.


The only region which differentiated FGFR2b (SwissProt reference P21802-3) and FGFR2c (SwissProt reference P21802) is between residues 313 and 354 of human FGFR2, as illustrated in the attachement of this email. Unfortunately, we do not currently haveany antibodies directed towards this region (for human or rat samples). Additionally, this region shares homology with several of the other FGFR2 isoforms (K-sam, K-sam-IIO2, K-sam-IIH1 etc)and even this sequencewould therefore not be specific for FGFR2b.


I am sorry that I could not be of more help. If you require any further information, please do not hesitate to contact us again.

Read More

Answer

Thank you for contacting us and your interest in our products.

The immunogen used to raise anti-FGFR2 antibody (ab10648) is of the sequence APGREKEITASPD which corresponds to amino acid residues 362-374 of human FGFR2. Based on this immunogen sequence, this antibodywill not differentiate between the two forms of FGFR2: FGFR2b (SwissProt reference P21802-3)or FGFR2c(SwissProt reference P21802)as both isoforms contain this sequence (as illustrated in the attachment of this email).

This antibody is covered by the Abpromise for the application and species of samples you wish to test. More information on the Abpromise can be found from the following link:

https://www.abcam.com/abpromise

I hope this information has been of help, if you require any further information please do not hesitate to contact us again.

Read More

Answer

Thanks for your email.
Earlier today I sent you and email containing instructions on returning an antibody to Abcam. Below, please find those same instructions:
===========
Included in this email are the instructions for returning your item/s to Abcam. When you return the goods, please ensure that you follow the procedures as below:
- Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today.
- Return all documents (or copies of) that arrived with the product (if they’re available – should be invoice, delivery note and datasheet)
- Please return via an overnight courier such as UPS, DHL, or FEDEX.
- Ship to the following address:
Attn: Returns ORDER REF: (_____)
Abcam Inc
One Kendall Square
Bldg 200, Suite B2304
Cambridge, MA 02139
Please be advised that a 20% restock fee will apply to this order. The most up-to-date information regarding your order, invoice, and credit note status is available online in your Abcam account information. If your order was placed by credit card, your refund will be issued to your credit card. If your order was placed by Purchase Order, all customers must submit a credit note with their invoice to their Accounts Payable department to be handled. The Accounts Payable must have a copy of the credit note so the original invoice can be updated to reflect the correct amount owed.
If you have any other questions please do not hesitate to contact us!
Email: US.orders@Abcam.com
Phone: 888-77-ABCAM(22226)
Fax: 866 739 9884
Customer Service hours of operation:
Monday-Friday 8:30 AM- 9:00PM EST

Read More

Answer

Thank you for contacting Abcam. Included in this email are the instructions for returning your item/s to Abcam. When you return the goods, please ensure that you follow the procedures as below:
- Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today.
- Return all documents (or copies of) that arrived with the product (if they’re available – should be invoice, delivery note and datasheet)
- Please return via an overnight courier such as UPS, DHL, or FEDEX.
- Ship to the following address:
Attn: Returns ORDER REF: (_____)
Abcam Inc
One Kendall Square
Bldg 200, Suite B2304
Cambridge, MA 02139
Please be advised that a 20% restock fee will apply to this order. The most up-to-date information regarding your order, invoice, and credit note status is available online in your Abcam account information. If your order was placed by credit card, your refund will be issued to your credit card. If your order was placed by Purchase Order, all customers must submit a credit note with their invoice to their Accounts Payable department to be handled. The Accounts Payable must have a copy of the credit note so the original invoice can be updated to reflect the correct amount owed.
If you have any other questions please do not hesitate to contact us!
Email: US.orders@Abcam.com
Phone: 888-77-ABCAM(22226)
Fax: 866 739 9884
Customer Service hours of operation:
Monday-Friday 8:30 AM- 9:00PM EST

Read More

Question
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacements on the following order numbers:
You will receive ab7192 to replace ab824
. You should receive this on Friday March 2.
You will receive ab119237 to replace ab10648.
You should receive this on or around Tuesday March 7 (it is back-ordered).
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

Read More

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up