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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-FGFR2 antibody [EPR5180] - BSA and Azide free (ab239970)

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  • Certificate of Compliance
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Immunoprecipitation - Anti-FGFR2 antibody [EPR5180] - BSA and Azide free (ab239970)
  • Anti-FGFR2 antibody [EPR5180] - BSA and Azide free (ab239970)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5180] to FGFR2 - BSA and Azide free
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Conjugation
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HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
Product image
Recombinant human FGFR2 protein (ab89755)

View more associated products

Overview

  • Product name

    Anti-FGFR2 antibody [EPR5180] - BSA and Azide free
    See all FGFR2 primary antibodies
  • Description

    Rabbit monoclonal [EPR5180] to FGFR2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FGFR2 aa 1-100 (extracellular). The exact sequence is proprietary.

  • Positive control

    • IP: T-47D whole cell lysate.
  • General notes

    Ab239970 is the carrier-free version of ab109372. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239970 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5180
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • FGF
    • FGF Receptors
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • FGF
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Cancer
    • Growth factors
    • FGF
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • Receptor tyrosine kinases
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Associated products

  • Alternative Versions

    • Anti-FGFR2 antibody [EPR5180] (ab109372)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 membrane extract lysate (ab29539)
    • HeLa membrane extract lysate (ab29547)
    • Jurkat membrane extract lysate (ab30130)
  • Recombinant Protein

    • Recombinant human FGFR2 protein (ab89755)

Applications

Our Abpromise guarantee covers the use of ab239970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 145 kDa (predicted molecular weight: 92 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Receptor for acidic and basic fibroblast growth factors.
    • Involvement in disease

      Defects in FGFR2 are the cause of Crouzon syndrome (CS) [MIM:123500]; also called craniofacial dysostosis type I (CFD1). CS is an autosomal dominant syndrome characterized by craniosynostosis (premature fusion of the skull sutures), hypertelorism, exophthalmos and external strabismus, parrot-beaked nose, short upper lip, hypoplastic maxilla, and a relative mandibular prognathism.
      Defects in FGFR2 are a cause of Jackson-Weiss syndrome (JWS) [MIM:123150]. JWS is an autosomal dominant craniosynostosis syndrome characterized by craniofacial abnormalities and abnormality of the feet: broad great toes with medial deviation and tarsal-metatarsal coalescence.
      Defects in FGFR2 are a cause of Apert syndrome (APRS) [MIM:101200]; also known as acrocephalosyndactyly type 1 (ACS1). APRS is a syndrome characterized by facio-cranio-synostosis, osseous and membranous syndactyly of the four extremities, and midface hypoplasia. The craniosynostosis is bicoronal and results in acrocephaly of brachysphenocephalic type. Syndactyly of the fingers and toes may be total (mitten hands and sock feet) or partial affecting the second, third, and fourth digits. Intellectual deficit is frequent and often severe, usually being associated with cerebral malformations.
      Defects in FGFR2 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly. Three subtypes of Pfeiffer syndrome have been described: mild autosomal dominant form (type 1); cloverleaf skull, elbow ankylosis, early death, sporadic (type 2); craniosynostosis, early demise, sporadic (type 3).
      Defects in FGFR2 are the cause of Beare-Stevenson cutis gyrata syndrome (BSCGS) [MIM:123790]. BSCGS is an autosomal dominant condition is characterized by the furrowed skin disorder of cutis gyrata, acanthosis nigricans, craniosynostosis, craniofacial dysmorphism, digital anomalies, umbilical and anogenital abnormalities and early death.
      Defects in FGFR2 are the cause of familial scaphocephaly syndrome (FSPC) [MIM:609579]; also known as scaphocephaly with maxillary retrusion and mental retardation. FSPC is an autosomal dominant craniosynostosis syndrome characterized by scaphocephaly, macrocephaly, hypertelorism, maxillary retrusion, and mild intellectual disability. Scaphocephaly is the most common of the craniosynostosis conditions and is characterized by a long, narrow head. It is due to premature fusion of the sagittal suture or from external deformation.
      Defects in FGFR2 are a cause of lacrimo-auriculo-dento-digital syndrome (LADDS) [MIM:149730]; also known as Levy-Hollister syndrome. LADDS is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. LADDS is an autosomal dominant syndrome characterized by aplastic/hypoplastic lacrimal and salivary glands and ducts, cup-shaped ears, hearing loss, hypodontia and enamel hypoplasia, and distal limb segments anomalies. In addition to these cardinal features, facial dysmorphism, malformations of the kidney and respiratory system and abnormal genitalia have been reported. Craniosynostosis and severe syndactyly are not observed.
      Defects in FGFR2 are the cause of Antley-Bixler syndrome (ABS) [MIM:207410]. ABS is a multiple congenital anomaly syndrome characterized by craniosynostosis, radiohumeral synostosis, midface hypoplasia, malformed ears, arachnodactyly and multiple joint contractures. ABS is a heterogeneous disorder and occurs with and without abnormal genitalia in both sexes.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P21802 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2263 Human
      • Entrez Gene: 14183 Mouse
      • Entrez Gene: 25022 Rat
      • Omim: 176943 Human
      • SwissProt: P21802 Human
      • SwissProt: P21803 Mouse
      • Unigene: 533683 Human
      • Unigene: 16340 Mouse
      • Unigene: 12732 Rat
      see all
    • Alternative names

      • bacteria-expressed kinase antibody
      • BBDS antibody
      • BEK antibody
      • BEK fibroblast growth factor receptor antibody
      • BFR1 antibody
      • CD332 antibody
      • CD332 antigen antibody
      • CEK3 antibody
      • CFD1 antibody
      • Craniofacial dysostosis 1 antibody
      • ECT1 antibody
      • FGF receptor antibody
      • FGFR 2 antibody
      • FGFR-2 antibody
      • Fgfr2 antibody
      • FGFR2_HUMAN antibody
      • Fibroblast growth factor receptor 2 antibody
      • Hydroxyaryl protein kinase antibody
      • Jackson Weiss syndrome antibody
      • JWS antibody
      • K SAM antibody
      • K-sam antibody
      • Keratinocyte growth factor receptor 2 antibody
      • Keratinocyte growth factor receptor antibody
      • KGFR antibody
      • KSAM antibody
      • protein tyrosine kinase, receptor like 14 antibody
      • soluble FGFR4 variant 4 antibody
      • TK14 antibody
      • TK25 antibody
      see all

    Images

    • Immunoprecipitation - Anti-FGFR2 antibody [EPR5180] - BSA and Azide free (ab239970)
      Immunoprecipitation - Anti-FGFR2 antibody [EPR5180] - BSA and Azide free (ab239970)

      Purified ab109372 at 1/40 dilution (2µg) immunoprecipitating FGFR2 in T-47D whole cell lysate.
      Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab109372 + T-47D whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109372 in T-47D whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 145 kDa
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109372).

    • Anti-FGFR2 antibody [EPR5180] - BSA and Azide free (ab239970)
      Anti-FGFR2 antibody [EPR5180] - BSA and Azide free (ab239970)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab239970? Please let us know so that we can cite the reference in this datasheet.

    ab239970 has not yet been referenced specifically in any publications.

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