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Fusion protein corresponding to Human FGFR4.
Our Abpromise guarantee covers the use of ab44971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC||Use at an assay dependent concentration.|
ab44971 Mouse monoclonal [5B5] to FGFR4 staining fibroblasts in normal human skin tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde and blocked in 10% BSA for 30 minutes at 21°C. The primary antibody was diluted 1/50 in PBT (PBS + 0.5% Tween 20 + 0.5% BSA) and incubated with sample for 30 minutes at 21°C. A HRP conjugated goat polyclonal to mouse IgG was used undiluted as secondary.
Formaldehyde-fixed, 5% Triton 100X permeabilized mouse primary airway cells labeling FGFR4 with ab44971 at 1/300 dilution in ICC/IF. 4% M.O.M Ig Blocking was used as a blocking agent for 1 hour at 20°C. Primary antibody was incubated at 4°C for 16 hours. Secondary antibody used was a biotin-conjugated rabbit polyclonal anti-mouse Ig at 1/100 dilution.
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