• Product name
    Anti-FGFR4 antibody [5B5]
    See all FGFR4 primary antibodies
  • Description
    Mouse monoclonal [5B5] to FGFR4
  • Host species
  • Specificity
    ab44971 detects fibroblasts.
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, ICC, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein corresponding to Human FGFR4.

  • Positive control
    • ICC/IF: Human BJ fibroblasts; mouse primary airway cells. IHC-Fr: Human skin fibroblasts. Flow: Human primary adult fibroblasts. IHC-P: Murine abdominal fibroblasts.



Our Abpromise guarantee covers the use of ab44971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
Flow Cyt 1/50.

See Abreview.




ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


ICC Use at an assay dependent concentration.
ICC/IF 1/50.
IHC-P 1/30.


  • Function
    Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19.
  • Tissue specificity
    Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization.
  • Cellular localization
    Membrane. Isoform 2 may be secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 334 antibody
    • CD334 antibody
    • CD334 antigen antibody
    • fc13h 10 antibody
    • fc13h10 antibody
    • Fgfr 4 antibody
    • FGFR-4 antibody
    • Fgfr4 antibody
    • FGFR4_HUMAN antibody
    • Fibroblast growth factor receptor 4 antibody
    • Hydroxyaryl protein kinase antibody
    • JTK 2 antibody
    • JTK2 antibody
    • MGC20292 antibody
    • Protein tyrosine kinase antibody
    • TKF antibody
    • Tyrosine kinase related to fibroblast growth factor receptor antibody
    • Tyrosylprotein kinase antibody
    see all


  • ab44971 (1/50) detecting fibroblast cells (red). Human BJ fibroblast cells were fixed in formaldehyde, permeabilized in 0.1% Triton and blocked in 8% BSA.
  • ab44971 Mouse monoclonal [5B5] to FGFR4 staining fibroblasts in normal human skin tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde and blocked in 10% BSA for 30 minutes at 21°C. The primary antibody was diluted 1/50 in PBT (PBS + 0.5% Tween 20 + 0.5% BSA) and incubated with sample for 30 minutes at 21°C. A HRP conjugated goat polyclonal to mouse IgG was used undiluted as secondary.

    See Abreview

  • ab44971 staining Human primary adult fibroblasts by Flow Cytometry. Cells were harvested with 1 min 0.05% trypsin treatment at 37°C in 5% FBS. Cells were fixed with paraformaldehyde and permeabilized with Triton X-100. The sample was incubated with the primary antibody, (1/50 in 5% FBS), for 20 minutes at 21°C. An Alexa Fluor®647-conjugated Goat polyclonal to mouse IgG (1/400) was used as secondary antibody.

    See Abreview

  • ab44971 Mouse monoclonal [5B5] to FGFR4 staining fibroblasts in murine abdominal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Formaldehyde-fixed, 5% Triton 100X permeabilized mouse primary airway cells labeling FGFR4 with ab44971 at 1/300 dilution in ICC/IF. 4% M.O.M Ig Blocking was used as a blocking agent for 1 hour at 20°C. Primary antibody was incubated at 4°C for 16 hours. Secondary antibody used was a biotin-conjugated rabbit polyclonal anti-mouse Ig at 1/100 dilution.

    See Abreview


This product has been referenced in:
  • Chen G  et al. Connexin-43 induces chemokine release from spinal cord astrocytes to maintain late-phase neuropathic pain in mice. Brain 137:2193-209 (2014). ICC/IF ; Mouse . Read more (PubMed: 24919967) »
  • Carmona R  et al. Cells derived from the coelomic epithelium contribute to multiple gastrointestinal tissues in mouse embryos. PLoS One 8:e55890 (2013). ICC/IF ; Mouse . Read more (PubMed: 23418471) »

See all 6 Publications for this product

Customer reviews and Q&As

Thank you for your inquiry.

Please find attached the Certificate of Compliance forlot 803659 ofab44971.

Please do not hesitate to contact me again with any further questions.

Thank you for contacting us. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our acc...

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer ph=6
Rat Tissue sections (Kidney)
Yes - PBS-Triton 0.1%, 10min, RT

Dr. Francois Daubeuf

Verified customer

Submitted Mar 11 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer ph=6
Pig Tissue sections (Lung Biopsy)
Lung Biopsy

Dr. Francois Daubeuf

Verified customer

Submitted Mar 11 2015

J’ai fait des recherches et j’ai trouvé des « Anti-Fibroblast activation protein » qui sont spécifiques aux cellules fibroblastes.

Ab53066, ab28244 et ab28246 sont garantis de fonctionner chez l’hum...

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Thank you for taking time to send us further details. I have successfully received the image sent, and could verify the lack of staining from ab44971.

The details provided will enable us to investigate this case and will provide us with vital...

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Thank you for contacting us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the...

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Thank you for contacting us.

Please have a look at the following reference we have on our website:

Liao IC & Leong KW Efficacy of engineered FVIII-producing skeletal muscle enhanced by growth factor-releasing co-axial electrosp...

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Thank you for your enquiry and your interest in our products.

I can confirm that the price of this antibody is €410.00. You can find all the necessary information on the on-line datasheet such as tested species, application, concentratio...

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Muchas gracias por contactarnos de nuevo.

Algunos de nuestros anticuerpos se suministran en gelatina, por lo que recomendamos almacenarlos a 4C.

Continuados ciclos de congelación / descongelación pueden dañar el...

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