Anti-FGFR4 antibody [5B5] (ab44971)
Key features and details
- Mouse monoclonal [5B5] to FGFR4
- Suitable for: ICC/IF, IHC-Fr, Flow Cyt, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-FGFR4 antibody [5B5]
See all FGFR4 primary antibodies -
Description
Mouse monoclonal [5B5] to FGFR4 -
Host species
Mouse -
Specificity
ab44971 detects fibroblasts. -
Tested applications
Suitable for: ICC/IF, IHC-Fr, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Fusion protein corresponding to Human FGFR4.
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Positive control
- ICC/IF: Human BJ fibroblasts; mouse primary airway cells. IHC-Fr: Human skin fibroblasts. Flow: Human primary adult fibroblasts. IHC-P: Murine abdominal fibroblasts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
5B5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab44971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (2) |
Use at an assay dependent concentration.
|
IHC-Fr | (2) |
Use at an assay dependent concentration.
|
Flow Cyt | (2) |
Use at an assay dependent concentration.
|
IHC-P | (7) |
Use at an assay dependent concentration.
|
Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19. -
Tissue specificity
Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsGlycosylated.
Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization. -
Cellular localization
Membrane. Isoform 2 may be secreted. - Information by UniProt
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Database links
- Entrez Gene: 2264 Human
- Entrez Gene: 14186 Mouse
- Omim: 134935 Human
- SwissProt: P22455 Human
- SwissProt: Q03142 Mouse
- Unigene: 165950 Human
- Unigene: 276715 Mouse
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Alternative names
- CD 334 antibody
- CD334 antibody
- CD334 antigen antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab44971 has been referenced in 12 publications.
- Castro-Viñuelas R et al. Generation and characterization of human induced pluripotent stem cells (iPSCs) from hand osteoarthritis patient-derived fibroblasts. Sci Rep 10:4272 (2020). PubMed: 32144293
- Chen YS et al. Ursodeoxycholic Acid Regulates Hepatic Energy Homeostasis and White Adipose Tissue Macrophages Polarization in Leptin-Deficiency Obese Mice. Cells 8:N/A (2019). PubMed: 30884843
- Hong SP et al. Single-cell transcriptomics reveals multi-step adaptations to endocrine therapy. Nat Commun 10:3840 (2019). PubMed: 31477698
- Kato T et al. Endothelial-mesenchymal transition in human atrial fibrillation. J Cardiol 69:706-711 (2017). PubMed: 27938856
- Sheipouri D et al. Characterisation of the kynurenine pathway in skin-derived fibroblasts and keratinocytes. J Cell Biochem 116:903-22 (2015). PubMed: 25639585