Overview

  • Product name
    Anti-FGFR4 antibody [5B5]
    See all FGFR4 primary antibodies
  • Description
    Mouse monoclonal [5B5] to FGFR4
  • Host species
    Mouse
  • Specificity
    ab44971 detects fibroblasts.
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, ICC, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein corresponding to Human FGFR4.

  • Positive control
    • ICC/IF: Human BJ fibroblasts; mouse primary airway cells. IHC-Fr: Human skin fibroblasts. Flow: Human primary adult fibroblasts. IHC-P: Murine abdominal fibroblasts.

Properties

Applications

Our Abpromise guarantee covers the use of ab44971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
Flow Cyt 1/50.

See Abreview.

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC Use at an assay dependent concentration.
ICC/IF 1/50.
IHC-P 1/30.

Target

  • Function
    Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19.
  • Tissue specificity
    Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Glycosylated.
    Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization.
  • Cellular localization
    Membrane. Isoform 2 may be secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 334 antibody
    • CD334 antibody
    • CD334 antigen antibody
    • fc13h 10 antibody
    • fc13h10 antibody
    • Fgfr 4 antibody
    • FGFR-4 antibody
    • Fgfr4 antibody
    • FGFR4_HUMAN antibody
    • Fibroblast growth factor receptor 4 antibody
    • Hydroxyaryl protein kinase antibody
    • JTK 2 antibody
    • JTK2 antibody
    • MGC20292 antibody
    • Protein tyrosine kinase antibody
    • TKF antibody
    • Tyrosine kinase related to fibroblast growth factor receptor antibody
    • Tyrosylprotein kinase antibody
    see all

Images

  • ab44971 (1/50) detecting fibroblast cells (red). Human BJ fibroblast cells were fixed in formaldehyde, permeabilized in 0.1% Triton and blocked in 8% BSA.
  • ab44971 Mouse monoclonal [5B5] to FGFR4 staining fibroblasts in normal human skin tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde and blocked in 10% BSA for 30 minutes at 21°C. The primary antibody was diluted 1/50 in PBT (PBS + 0.5% Tween 20 + 0.5% BSA) and incubated with sample for 30 minutes at 21°C. A HRP conjugated goat polyclonal to mouse IgG was used undiluted as secondary.

    See Abreview

  • ab44971 staining Human primary adult fibroblasts by Flow Cytometry. Cells were harvested with 1 min 0.05% trypsin treatment at 37°C in 5% FBS. Cells were fixed with paraformaldehyde and permeabilized with Triton X-100. The sample was incubated with the primary antibody, (1/50 in 5% FBS), for 20 minutes at 21°C. An Alexa Fluor®647-conjugated Goat polyclonal to mouse IgG (1/400) was used as secondary antibody.

    See Abreview

  • ab44971 Mouse monoclonal [5B5] to FGFR4 staining fibroblasts in murine abdominal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Formaldehyde-fixed, 5% Triton 100X permeabilized mouse primary airway cells labeling FGFR4 with ab44971 at 1/300 dilution in ICC/IF. 4% M.O.M Ig Blocking was used as a blocking agent for 1 hour at 20°C. Primary antibody was incubated at 4°C for 16 hours. Secondary antibody used was a biotin-conjugated rabbit polyclonal anti-mouse Ig at 1/100 dilution.

    See Abreview

References

This product has been referenced in:
  • Chen G  et al. Connexin-43 induces chemokine release from spinal cord astrocytes to maintain late-phase neuropathic pain in mice. Brain 137:2193-209 (2014). ICC/IF ; Mouse . Read more (PubMed: 24919967) »
  • Carmona R  et al. Cells derived from the coelomic epithelium contribute to multiple gastrointestinal tissues in mouse embryos. PLoS One 8:e55890 (2013). ICC/IF ; Mouse . Read more (PubMed: 23418471) »
See all 6 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-10 of 14 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer ph=6
Sample
Rat Tissue sections (Kidney)
Specification
Kidney
Permeabilization
Yes - PBS-Triton 0.1%, 10min, RT
Fixative
Formaldehyde

Dr. Francois Daubeuf

Verified customer

Submitted Mar 11 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer ph=6
Sample
Pig Tissue sections (Lung Biopsy)
Specification
Lung Biopsy
Permeabilization
No
Fixative
Formaldehyde

Dr. Francois Daubeuf

Verified customer

Submitted Mar 11 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human tissue array)
Specification
human tissue array
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9.0
Permeabilization
Yes - 0.25%Triton x-100 PBS, RT, 15min
Blocking step
Dakocytomation X0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C

Hongwei Shao

Verified customer

Submitted Sep 19 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (dermal cross section)
Specification
dermal cross section
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
Yes - triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Mrs. Shelley Lietzau-Mourer

Verified customer

Submitted Jan 21 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citronensäure+Natriumcitrat
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Dec 23 2011

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Breast)
Specification
Breast
Fixative
Formaldehyde
Permeabilization
Yes - Triton X-100 0.1%
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C

Dr. Rene Villadsen

Verified customer

Submitted Jul 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (Primary breast tissue)
Specification
Primary breast tissue
Fixative
Methanol
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C

Dr. Rene Villadsen

Verified customer

Submitted Jul 05 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH=6
Permeabilization
No
Blocking step
Sheep Anti-mouse IgG in PBS-BSA1%-Gelatin0,1% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 25°C

Dr. Francois Daubeuf

Verified customer

Submitted May 26 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Primary airway cells in culture)
Specification
Primary airway cells in culture
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% triton x100
Blocking step
M.O.M Ig Blocking as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C

Dr. Francois Daubeuf

Verified customer

Submitted Mar 04 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (primary adult fibroblasts)
Specification
primary adult fibroblasts
Preparation
Cell harvesting/tissue preparation method: Harvesting with 1min 0.05% trypsin treatment at 37oC
Sample buffer: 5% FBS
Fixation
Paraformaldehyde
Permeabilization
Yes - Triton X-100

Abcam user community

Verified customer

Submitted Apr 20 2010

1-10 of 14 Abreviews

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up