• Product name

  • Description

    Rabbit polyclonal to FGFR4
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human FGFR4.

    Read Abcam's proprietary immunogen policy (Peptide available as ab42267.)

  • Positive control

    • ab41948 gave a positive result in MDA MB 361 whole cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human liver cancer.



Our Abpromise guarantee covers the use of ab41948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
IHC-P Use a concentration of 5 µg/ml.


  • Function

    Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19.
  • Tissue specificity

    Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational

    Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization.
  • Cellular localization

    Membrane. Isoform 2 may be secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 334 antibody
    • CD334 antibody
    • CD334 antigen antibody
    • fc13h 10 antibody
    • fc13h10 antibody
    • Fgfr 4 antibody
    • FGFR-4 antibody
    • Fgfr4 antibody
    • FGFR4_HUMAN antibody
    • Fibroblast growth factor receptor 4 antibody
    • Hydroxyaryl protein kinase antibody
    • JTK 2 antibody
    • JTK2 antibody
    • MGC20292 antibody
    • Protein tyrosine kinase antibody
    • TKF antibody
    • Tyrosine kinase related to fibroblast growth factor receptor antibody
    • Tyrosylprotein kinase antibody
    see all


  • Anti-FGFR4 antibody (ab41948) at 1 µg/ml + MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 89 kDa
    Observed band size: 89 kDa
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.

  • ICC/IF image of ab41948 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab41948, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.

  • IHC image of FGFR4 staining in Human liver cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41948, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Wei W  et al. Prognostic implications of fibroblast growth factor receptor 4 polymorphisms in primary breast cancer. Mol Carcinog 57:988-996 (2018). Read more (PubMed: 29603419) »
  • Mutsaers HA  et al. Switch in FGFR3 and -4 expression profile during human renal development may account for transient hypercalcemia in patients with Sotos syndrome due to 5q35 microdeletions. J Clin Endocrinol Metab 99:E1361-7 (2014). Read more (PubMed: 24670087) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Western blot
Human Cell lysate - whole cell (A549)
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 27 2009

Western blot
Mouse Tissue lysate - whole (Lung, Heart, Skeletal muscle)
Loading amount
20 µg
Lung, Heart, Skeletal muscle
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Antibody Solutions

Verified customer

Submitted Jun 26 2008

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